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Study of the metabolic alterations in patulin-induced neoplastic transformation in normal intestinal cells
- Source :
- Toxicol Res (Camb)
- Publication Year :
- 2021
- Publisher :
- Oxford University Press (OUP), 2021.
-
Abstract
- Several surveillance studies have reported significantly high level of patulin (PAT), mycotoxin in fruit juices suggesting the possible exposure to human. In vitro studies have showed that PAT can alter the permeability, ion transport and modulates tight junction of intestine. In real scenario, human can be exposed with low levels of PAT for longer duration through different fruits and their products. Hence, keeping this possibility in view, we conducted a study where normal intestinal cells were exposed with non-toxic levels of PAT for longer duration and found that PAT exposure causes cancer-like properties in normal intestinal cells. It is a well-known fact that cancer cells rewired their metabolism for cell growth and survival and metabolites closely depict the phenotypic properties of cells. Here, metabolomic study was performed in the PAT transformed and passage matched non-transformed cells using 1H HRMAS NMR. We have identified 12 significantly up-regulated metabolites, which, interestingly, were majorly amino acids, suggesting that PAT-induced pre-cancerous cells are involved in acquirement of nutrients for high protein turn-over. Furthermore, pathway analysis of metabolomics data indicated that aminoacyl tRNA biosynthesis, D-glutamate metabolism, glyoxylate and dicarboxylate metabolism and nitrogen metabolism were majorly hampered in PAT-induced pre-cancerous properties in normal intestinal cells.
- Subjects :
- Paper
0303 health sciences
animal structures
Cell growth
Health, Toxicology and Mutagenesis
Glyoxylate and dicarboxylate metabolism
Metabolism
Toxicology
In vitro
Patulin
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Metabolomics
chemistry
Biochemistry
030220 oncology & carcinogenesis
Cancer cell
cardiovascular system
Neoplastic transformation
030304 developmental biology
Subjects
Details
- ISSN :
- 20454538
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Toxicology Research
- Accession number :
- edsair.doi.dedup.....abf230546cac83095820ae8f44d81500