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Identification of MRP-8 (calgranulin A) as a major responsive protein in chronic periodontitis
- Source :
- The Journal of Pathology. 192:540-544
- Publication Year :
- 2000
- Publisher :
- Wiley, 2000.
-
Abstract
- The purpose of the study was to analyse how the protein composition of the inflammatory exudate associated with chronic periodontitis differed from the exudate in periodontal health. Gingival crevicular fluid (GCF) was collected from sites with chronic periodontal inflammation and from non-diseased sites in healthy control subjects. Microbore HPLC analysis revealed one major difference in GCF protein profiles between healthy controls and periodontitis patients. The protein enhanced in periodontitis patients was identified as migration inhibitory factor-related protein-8 (MRP-8) by a combination of N-terminal amino acid sequencing, mass spectrometry, and SDS-PAGE. Together, these data demonstrate, for the first time, the presence of monomeric MRP-8 in an inflammatory exudate. Whether monomeric MRP-8 is a unique feature of chronic periodontal inflammation is not yet clear, but the chemotactic properties of this peptide support a functional role for MRP-8 in periodontal inflammation.
- Subjects :
- Adult
Male
Exudate
Spectrometry, Mass, Electrospray Ionization
Molecular Sequence Data
Inflammation
Peptide
Pathology and Forensic Medicine
Pathogenesis
stomatognathic system
medicine
Humans
Calgranulin A
Amino Acid Sequence
Periodontitis
Chromatography, High Pressure Liquid
chemistry.chemical_classification
business.industry
Calcium-Binding Proteins
Chemotaxis
Gingival Crevicular Fluid
Middle Aged
medicine.disease
Chronic periodontitis
Amino acid
chemistry
Chronic Disease
Immunology
Electrophoresis, Polyacrylamide Gel
Female
medicine.symptom
business
Subjects
Details
- ISSN :
- 10969896 and 00223417
- Volume :
- 192
- Database :
- OpenAIRE
- Journal :
- The Journal of Pathology
- Accession number :
- edsair.doi.dedup.....abedb95a60a29f6cf2f0c30957d8a161