Back to Search
Start Over
Detection of a hypersensitive reaction in the chestnut hybrid 'Bouche de Bétizac' infested by Dryocosmus kuriphilus Yasumatsu
- Source :
- Plant physiology and biochemistry : PPB. 60
- Publication Year :
- 2012
-
Abstract
- The purpose of the study was the identification of the mechanisms of resistance to Dryocosmus kuriphilus Yasumatsu in the hybrid-resistant cultivar 'Bouche de Betizac' (Castanea sativa × Castanea crenata). Larvae and eggs of the insect are found in the buds of this cultivar at the end of winter, but there is no gall development after budburst. The hypothesis of the presence of a hypersensitive reaction (HR) in the buds was tested using diaminobenzidine (DAB) to detect H(2)O(2) and by Real Time PCR (RT-PCR) to evaluate the expression of a germin-like protein gene. HR in plants is elicited by the production of reactive oxygen compounds, such as H(2)O(2), and results in the programmed cell death. The DAB test was applied to buds of 'Bouche de Betizac' and of the susceptible cultivar 'Madonna' (C. sativa) at different stages of budburst. The DAB staining produced brown areas in the swelling buds of 'Bouche de Betizac', indicating the presence of H(2)O(2). On the contrary, all uninfested buds, as well as the infested buds of 'Madonna', appeared whitish. Papers report that germin and germin-like proteins (GLP) with oxalate oxidase activity are discrete markers of stress-responsive gene products. A strong expression of the chestnut GLP gene was detected by RT-PCR at bud swelling in infested 'Bouche de Betizac' buds but not in 'Madonna' ones. The results support the hypothesis of the occurrence of an HR in 'Bouche de Betizac' as response to the cynipid infestation, resulting in cell and larvae death.
- Subjects :
- Physiology
media_common.quotation_subject
GLP
Wasps
Oxalate oxidase activity
Apoptosis
Plant Science
Insect
medicine.disease_cause
Fagaceae
Real-Time Polymerase Chain Reaction
Germin-like protein
Host-Parasite Interactions
Gene Expression Regulation, Plant
Botany
Infestation
Castanea sativa Mill
Genetics
medicine
Gall
DAB
Animals
Cultivar
Diaminobenzidine
Cynipid
media_common
Glycoproteins
Plant Diseases
Plant Proteins
Larva
biology
Cell Death
Chimera
Hydrogen Peroxide
biology.organism_classification
Dryocosmus kuriphilus
Shoot
Disease Susceptibility
Oxidoreductases
Plant Shoots
Subjects
Details
- ISSN :
- 18732690
- Volume :
- 60
- Database :
- OpenAIRE
- Journal :
- Plant physiology and biochemistry : PPB
- Accession number :
- edsair.doi.dedup.....abde7cf1cdb4025fb288017e80e4e3df