Additional file 1 of Atg7 deficiency in microglia drives an altered transcriptomic profile associated with an impaired neuroinflammatory response

Additional file 1: Figure S1. Reduction in Atg7 expression decreases the ability of BV2 microglia to undergo autophagy. a) Immunoblot analysis and b) quantification of LC3-I and LC3-II expression versus ACTB in shCtrl BV2 cells versus shAtg7 BV2 cells. Cells were cultured in EBSS medium to induce starvation, or stimulated with 250 nM Torin1 for 2 h, to induce autophagy. Treatment with BafA1 (40 nM), late inhibitor of autophagy, before sample collection was used to block the autophagic flux. c) Comparison of Nos2 mRNA expression measured by RT-qPCR in shAtg7 and shCtrl BV2 cells. Cells were pre-treated with BafA1 (40 nM) for 30 min and then treated with LPS (100 ng/ml) for 3 h. Values are a mean of 3 (c) or 4 (b) independent experiments �� SEM and considered significant for *p