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A Recombinant Insect-Specific alpha-Toxin of Buthus occitanus tunetanus Scorpion Confers Protection Against Homologous Mammal Toxins
- Source :
- European Journal of Biochemistry, European Journal of Biochemistry, 1996, 238 (3), pp.653-660. ⟨10.1111/j.1432-1033.1996.0653w.x⟩
- Publication Year :
- 1996
- Publisher :
- Wiley, 1996.
-
Abstract
- International audience; We have constructed a cDNA library from venom glands of the scorpion Buthus occitanus tunetanus and cloned a DNA sequence that encodes an alpha-toxin. This clone was efficiently expressed in Escherichia coli as a fusion protein with two Ig-binding (Z) domains of protein A from Staphylococcus aureus. After CNBr treatment of the fusion protein and HPLC purification, we obtained approximately 1 mg recombinant apha-toxin/l bacterial culture. The toxin, called Bot XIV, displays no toxicity towards mammals but is active towards insects as shown by its paralytic activity against Blatella germanica cockroach and by electrophysiological studies on Periplaneta americana cockroaches. The Bot XIV protein fused to two Z domains is highly immunogenic in mice and induces production of antisera that specifically recognize and neutralize highly toxic components that had been injected into mice. This fusion protein could be very useful for development of potent protective antisera against scorpion venoms.
- Subjects :
- MESH: Protein Precursors
[SDV]Life Sciences [q-bio]
Scorpion Venoms
Cockroaches
Venom
MESH: Amino Acid Sequence
MESH: Base Sequence
Biochemistry
law.invention
MESH: Cross Reactions
Mice
MESH: Scorpion Venoms
law
MESH: Animals
Cloning, Molecular
Mammals
Mice, Inbred BALB C
biology
MESH: Antibody Formation
MESH: DNA Probes
Recombinant DNA
MESH: Immunization
MESH: Cockroaches
MESH: Type C Phospholipases
DNA Probes
Periplaneta
MESH: Axons
DNA, Complementary
animal structures
Recombinant Fusion Proteins
Molecular Sequence Data
MESH: Mice, Inbred BALB C
Cross Reactions
complex mixtures
MESH: Mammals
Complementary DNA
MESH: Recombinant Fusion Proteins
Animals
MESH: Cloning, Molecular
Amino Acid Sequence
Protein Precursors
MESH: Mice
MESH: Molecular Sequence Data
Base Sequence
MESH: DNA, Complementary
biology.organism_classification
Fusion protein
Molecular biology
Axons
Type C Phospholipases
Antibody Formation
biology.protein
Immunization
Buthus occitanus
Protein A
Subjects
Details
- ISSN :
- 14321033, 00142956, and 14321327
- Volume :
- 238
- Database :
- OpenAIRE
- Journal :
- European Journal of Biochemistry
- Accession number :
- edsair.doi.dedup.....abcd192c1d72c331a1c943d538e20b44