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Analysis of neofructooligosaccharides production mediated by the extracellular β-fructofuranosidase from Xanthophyllomyces dendrorhous
- Source :
- Digital.CSIC. Repositorio Institucional del CSIC, instname
- Publication Year :
- 2012
- Publisher :
- Elsevier, 2012.
-
Abstract
- The extracellular β-fructofuranosidase Xd-INV from the yeast Xanthophyllomyces dendrorhous mainly synthesizes the neo-fructooligosaccharides (neo-FOS) neokestose and neonystose. This enzyme is a glycoprotein with a content of 59-67% N-linked carbohydrates and an estimated molecular mass of 160-200. kDa. The extent level of glycosylation affects the thermal behaviour of the enzyme but not its hydrolase and transferase activities, which are optimal at 60-70 °C. The neo-FOS yield of this enzyme was increased from 40 to 168. g/L when the sucrose concentration increased from 420 to 600. g/L and when the reaction was carried out at 60 °C. The neo-FOS levels obtained (168. g/L) in this work are the largest reported for any microbial β-fructofuranosidase. © 2012 Elsevier Ltd.
- Subjects :
- Sucrose
Glycosylation
Time Factors
Environmental Engineering
Hydrolases
Neofructooligosaccharides
Oligosaccharides
Bioengineering
chemistry.chemical_compound
Transferases
Hydrolase
Extracellular
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Waste Management and Disposal
Chromatography, High Pressure Liquid
chemistry.chemical_classification
beta-Fructofuranosidase
Molecular mass
Renewable Energy, Sustainability and the Environment
Basidiomycota
Temperature
General Medicine
Yeast
Kinetics
Xanthophyllomyces dendrorhous
Extracellular b-fructofuranosidase
Enzyme
Prebiotics
chemistry
Biochemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Biocatalysis
Electrophoresis, Polyacrylamide Gel
Extracellular Space
Glycoprotein
Trisaccharides
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Digital.CSIC. Repositorio Institucional del CSIC, instname
- Accession number :
- edsair.doi.dedup.....abcc4e79d692ac3bd2cbaa0851b2eeb5