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Micro‐ and nano‐tomography analysis of mouse soleus muscle using radiation
- Source :
- Microscopy Research and Technique. 84:2685-2693
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- In this study, we analyze radiation images of muscle structure of mice soleus muscles using radiation source-based microtomography and nanotomography. Soleus muscle samples were collected for analysis from 8-week-old male Institute of Cancer Research mice. First, phase-contrast X-ray microtomography was employed in these experiments. Then to obtain images with excellent contrast, imaging was performed using monochromatic light with excellent transmission power. To analyze additional muscle structures in higher magnification images than these images, nanotomography was performed, which facilitated obtaining high-magnification and high-resolution images. Muscle tissue microstructures were confirmed through three-dimensional images obtained from phase-contrast X-ray microtomography. Thus, the muscle tissue's overall shape at microscopic level can be captured. Additionally, a single muscle fiber was examined using hard X-ray nano-imaging, through which we could observe the alignment of countless myofibrils, that is, actin and myosin filaments in the muscle fibers. Thus, the methodology adopted here proved to be advantageous in analyzing the muscle tissue's overall structure with microtomography and in observing the myofibrils in detail using nanotomography.
- Subjects :
- Male
Muscle tissue
Histology
X-ray microtomography
Materials science
Magnification
02 engineering and technology
Mice
03 medical and health sciences
Imaging, Three-Dimensional
0302 clinical medicine
Myosin
medicine
Animals
Microscopy, Phase-Contrast
Muscle, Skeletal
Instrumentation
Actin
Soleus muscle
X-Ray Microtomography
030206 dentistry
021001 nanoscience & nanotechnology
Actins
Medical Laboratory Technology
medicine.anatomical_structure
Tomography
Anatomy
0210 nano-technology
Myofibril
Biomedical engineering
Subjects
Details
- ISSN :
- 10970029 and 1059910X
- Volume :
- 84
- Database :
- OpenAIRE
- Journal :
- Microscopy Research and Technique
- Accession number :
- edsair.doi.dedup.....ababc87baf6e691a7ba64d983c7e8f01