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Feeding practice influences gut microbiome composition in very low birth weight preterm infants and the association with oxidative stress: A prospective cohort study
- Source :
- Free radical biologymedicine. 142
- Publication Year :
- 2018
-
Abstract
- Knowledge about the development of the preterm infant gut microbiota is emerging and is critical to their health. Very-low-birth-weight (VLBW; birth weight,1500 g) infants usually have special dietary needs while showing increased oxidative stress related to intensive care. This prospective cohort study assessed the effect of feeding practice on gut microbiome development and oxidative stress in preterm infants. Fecal samples were collected from each infant in the early (1-2 weeks of enteral feeding) and late (2-4 weeks of enteral feeding) feeding stages. We performed high-throughput sequencing of V3-V4 regions of the 16S rRNA gene to analyze the fecal microbiome composition of 20 VLBW preterm infants and to determine the association of gut bacterial composition with feeding practice using an oxidative stress marker (urinary F2-isoprostane). Our results showed that feeding practices in the late stage significantly influenced the gut microbiome composition and oxidative stress in preterm infants. Preterm infants fed human milk + human milk fortifier and only formula diets showed a significant increase in F2-isoprostane levels (P 0.05) compared with those fed human milk + formula diet. The gut microbiome of the infants fed the human milk + Human milk fortifier diet showed the lower relative abundance of Veillonella (P 0.05) compared with that of the infants fed the human milk + formula diet. The gut microbiome of the infants fed the only formula diet showed the lowest microbial diversity and the highest relative abundance of Terrisporobacter (P 0.05) compared with the gut microbiome of the infants fed the other diets. Correlation network analysis showed that urinary F2-isoprostane level was positively correlated with Terrisporobacter and Enterobacteriaceae abundance (P 0.05) in the preterm infants. In conclusion, these data suggest that feeding practice affects the bacterial diversity and composition in the gut microbiome and is associated with oxidative stress in VLBW preterm infants.
- Subjects :
- 0301 basic medicine
Male
Birth weight
Physiology
Gestational Age
Gut flora
medicine.disease_cause
Biochemistry
Enteral administration
Veillonella
03 medical and health sciences
Feces
0302 clinical medicine
Enteral Nutrition
Enterobacteriaceae
Physiology (medical)
RNA, Ribosomal, 16S
Medicine
Humans
Infant, Very Low Birth Weight
Microbiome
Prospective Studies
Prospective cohort study
2. Zero hunger
Clostridiales
F2-Isoprostanes
biology
Milk, Human
business.industry
Infant, Newborn
Computational Biology
High-Throughput Nucleotide Sequencing
Infant
biology.organism_classification
Infant Formula
3. Good health
Diet
Gastrointestinal Microbiome
Low birth weight
Oxidative Stress
030104 developmental biology
Female
medicine.symptom
business
030217 neurology & neurosurgery
Oxidative stress
Biomarkers
Infant, Premature
Subjects
Details
- ISSN :
- 18734596
- Volume :
- 142
- Database :
- OpenAIRE
- Journal :
- Free radical biologymedicine
- Accession number :
- edsair.doi.dedup.....aba01878509f3cd7fa1fa6585833e1b0