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Development of nonfibrotic left ventricular hypertrophy in an ANG II-induced chronic ovine hypertension model
- Source :
- Physiological Reports
- Publication Year :
- 2016
- Publisher :
- Wiley, 2016.
-
Abstract
- Hypertension is a major risk factor for many cardiovascular diseases and leads to subsequent concomitant pathologies such as left ventricular hypertrophy (LVH). Translational approaches using large animals get more important as they allow the use of standard clinical procedures in an experimental setting. Therefore, the aim of this study was to establish a minimally invasive ovine hypertension model using chronic angiotensin II (ANG II) treatment and to characterize its effects on cardiac remodeling after 8 weeks. Sheep were implanted with osmotic minipumps filled with either vehicle control ( n = 7) or ANG II ( n = 9) for 8 weeks. Mean arterial blood pressure in the ANG II‐treated group increased from 87.4 ± 5.3 to 111.8 ± 6.9 mmHg ( P = 0.00013). Cardiovascular magnetic resonance imaging showed an increase in left ventricular mass from 112 ± 12.6 g to 131 ± 18.7 g after 7 weeks ( P = 0.0017). This was confirmed by postmortem measurement of left ventricular wall thickness which was higher in ANG II‐treated animals compared to the control group (18 ± 4 mm vs. 13 ± 2 mm, respectively, P = 0.002). However, ANG II‐treated sheep did not reveal any signs of fibrosis or inflammatory infiltrates as defined by picrosirius red and H&E staining on myocardial full thickness paraffin sections of both atria and ventricles. Measurements of plasma high‐sensitivity C‐reactive protein and urinary 8‐iso‐prostaglandin F 2 α were inconspicuous in all animals. Furthermore, multielectrode surface mapping of the heart did not show any differences in epicardial conduction velocity and heterogeneity. These data demonstrate that chronic ANG II treatment using osmotic minipumps presents a reliable, minimally invasive approach to establish hypertension and nonfibrotic LVH in sheep.
- Subjects :
- Cardiovascular Conditions, Disorders and Treatments
0301 basic medicine
medicine.medical_specialty
hypertension
Physiology
Heart Ventricles
Urinary system
Blood Pressure
030204 cardiovascular system & hematology
Left ventricular hypertrophy
Nerve conduction velocity
03 medical and health sciences
0302 clinical medicine
Risk Factors
Fibrosis
Physiology (medical)
Internal medicine
medicine
Animals
Vasoconstrictor Agents
CMR
Original Research
Sheep
Ventricular Remodeling
medicine.diagnostic_test
business.industry
Angiotensin II
Heart
Magnetic resonance imaging
medicine.disease
Magnetic Resonance Imaging
left ventricular hypertrophy
030104 developmental biology
Blood pressure
Concomitant
Models, Animal
Cardiology
Hypertrophy, Left Ventricular
Autopsy
business
Subjects
Details
- ISSN :
- 2051817X
- Volume :
- 4
- Database :
- OpenAIRE
- Journal :
- Physiological Reports
- Accession number :
- edsair.doi.dedup.....ab86e67c69c296b3c7e4942dfb342cb9