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Establishment of sister chromatid cohesion at the S. cerevisiae replication fork
- Source :
- Molecular Cell, Molecular Cell, Elsevier, 2006, 23 (6), pp.787-799. ⟨10.1016/j.molcel.2006.08.018⟩
- Publication Year :
- 2006
- Publisher :
- HAL CCSD, 2006.
-
Abstract
- Two identical sister copies of eukaryotic chromosomes are synthesized during S phase. To facilitate their recognition as pairs for segregation in mitosis, sister chromatids are held together from their synthesis onward by the chromosomal cohesin complex. Replication fork progression is thought to be coupled to establishment of sister chromatid cohesion, facilitating identification of replication products, but evidence for this has remained circumstantial. Here we show that three proteins required for sister chromatid cohesion, Eco1, Ctf4, and Ctf18, are found at, and Ctf4 travels along chromosomes with, replication forks. The ring-shaped cohesin complex is loaded onto chromosomes before S phase in an ATP hydrolysis-dependent reaction. Cohesion establishment during DNA replication follows without further cohesin recruitment and without need for cohesin to re-engage an ATP hydrolysis motif that is critical for its initial DNA binding. This provides evidence for cohesion establishment in the context of replication forks and imposes constraints on the mechanism involved.
- Subjects :
- DNA Replication
Saccharomyces cerevisiae Proteins
Cohesin complex
Chromosomal Proteins, Non-Histone
Amino Acid Motifs
Cell Cycle Proteins
Saccharomyces cerevisiae
Biology
Chromatids
S Phase
03 medical and health sciences
0302 clinical medicine
Control of chromosome duplication
Acetyltransferases
Proliferating Cell Nuclear Antigen
Sister chromatids
DNA, Fungal
Molecular Biology
030304 developmental biology
Cohesin loading
Genetics
Adenosine Triphosphatases
0303 health sciences
[SDV.GEN]Life Sciences [q-bio]/Genetics
Cohesin
Models, Genetic
Nuclear Proteins
Cell Biology
Establishment of sister chromatid cohesion
DNA-Binding Proteins
Origin recognition complex
biological phenomena, cell phenomena, and immunity
Separase
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 10972765
- Database :
- OpenAIRE
- Journal :
- Molecular Cell, Molecular Cell, Elsevier, 2006, 23 (6), pp.787-799. ⟨10.1016/j.molcel.2006.08.018⟩
- Accession number :
- edsair.doi.dedup.....ab802f8a24339e0a0761e295d8debd73