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Participation of autophagy in lung ischemia–reperfusion injury in vivo
- Source :
- Journal of Surgical Research. 182:e79-e87
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- Background In response to stress, autophagy is activated and may present as a cell survival mechanism or lead to cell death. Although there has been some progress in studying the function of autophagy in the ischemia–reperfusion (I/R) injury of other organs, little is known about the role autophagy plays in lung I/R injury. Methods A lung I/R injury model in rats was developed to explore the level of autophagy in lung I/R injury. An inhibitor of autophagy (3-methyladenine [3-MA]) was injected before ischemia to study the role autophagy plays in lung I/R injury. Results The data indicated that the autophagic flux was elevated during the ischemia period, and was enhanced significantly during reperfusion. Inhibition of autophagy by 3-MA ameliorated lung I/R injury, as indicated by a reduced lung wet/dry ratio, myeloperoxidase activity, and malondialdehyde concentrations. 3-MA pretreatment also reduced cleaved caspase-3 and apoptosis in the lung, as indicated by the terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Conclusions The results demonstrated that autophagy was involved in the lung I/R pathophysiological process, and it may be a scathing factor in lung I/R injury.
- Subjects :
- Male
Programmed cell death
Pathology
medicine.medical_specialty
Ischemia
Apoptosis
Pharmacology
Biology
Rats, Sprague-Dawley
Autophagy
medicine
Animals
Lung
Caspase 3
Adenine
respiratory system
medicine.disease
Rats
respiratory tract diseases
Transplantation
medicine.anatomical_structure
Terminal deoxynucleotidyl transferase
Reperfusion Injury
Female
Surgery
Reperfusion injury
Subjects
Details
- ISSN :
- 00224804
- Volume :
- 182
- Database :
- OpenAIRE
- Journal :
- Journal of Surgical Research
- Accession number :
- edsair.doi.dedup.....ab62962c2173e1bb73eff1e359731038