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Photoinduced Photosensitizer–Antibody Conjugates Kill HIV Env-Expressing Cells, Also Inactivating HIV
- Source :
- ACS Omega, Vol 6, Iss 25, Pp 16524-16534 (2021), Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP, ACS Omega
- Publication Year :
- 2021
- Publisher :
- American Chemical Society (ACS), 2021.
-
Abstract
- HIV-infected cells persist for decades in patients administered with antiretroviral therapy (ART). Meanwhile, an alarming surge in drug-resistant HIV viruses has been occurring. Addressing these issues, we propose the application of photoimmunotherapy (PIT) against not only HIV Env-expressing cells but also HIV. Previously, we showed that a human anti-gp41 antibody (7B2) conjugated to cationic or anionic photosensitizers (PSs) could specifically target and kill the HIV Env-expressing cells. Here, our photolysis studies revealed that the binding of photoimmunoconjugates (PICs) on the membrane of HIV Env-expressing cells is sufficient to induce necrotic cell death due to physical damage to the membrane by singlet oxygen, which is independent of the type of PSs. This finding persuaded us to study the virus photoinactivation of PICs using two HIV-1 strains, X4 HIV-1 NL4-3 and JR-CSF virus. We observed that the PICs could destroy the viral strains, probably via physical damage on the HIV envelope. In conclusion, we report the application of PIT as a possible dual-tool for HIV immunotherapy and ART by killing HIV-expressing cells and cell-free HIV, respectively.
- Subjects :
- biology
Chemistry
viruses
General Chemical Engineering
medicine.medical_treatment
Human immunodeficiency virus (HIV)
virus diseases
Photoimmunotherapy
General Chemistry
Immunotherapy
medicine.disease_cause
Virology
Article
TERAPIA FOTODINÂMICA
Virus
0904 Chemical Engineering, 0912 Materials Engineering
medicine
biology.protein
Photosensitizer
In patient
Antibody
QD1-999
Conjugate
Subjects
Details
- ISSN :
- 24701343
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- ACS Omega
- Accession number :
- edsair.doi.dedup.....ab55b9fbe9e09cf56ad5dcce3ae98732