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Salmonella enterica Serovar Typhimurium Expressing Mutant Lipid A with Decreased Endotoxicity Causes Maturation of Murine Dendritic Cells
- Source :
- Infection and Immunity. 71:6132-6140
- Publication Year :
- 2003
- Publisher :
- American Society for Microbiology, 2003.
-
Abstract
- A major Salmonella component involved in cellular activation is the lipopolysaccharide (LPS) molecule which can act as a dendritic cell (DC) stimulator. The structure of the lipid A domain of the LPS molecule dictates its immunostimulatory capacity with various cell types. In this study, the role of lipid A as an integral component of Salmonella in stimulating murine DCs was studied by using a Salmonella enterica serovar Typhimurium lpxM mutant with defective lipid A. This study revealed that a mutation in lpxM did not significantly affect the ability of bacteria to activate DCs. Although the lpxM mutant less tumor necrosis factor alpha, interleukin-1β, and inducible nitric oxide synthase than the parental strain, this was only seen at lower multiplicities of infection (MOIs). Both strains upregulated surface molecule expression on DCs and augmented the T-cell-stimulating capacity of these cells in an MOI-independent manner. Thus, the lpxM mutation did not appear to affect the stimulatory capacity of the Salmonella mutant.
- Subjects :
- Salmonella typhimurium
Salmonella
Lipopolysaccharide
Immunology
Mutant
Nitric Oxide Synthase Type II
Biology
medicine.disease_cause
Microbiology
Lipid A
Mice
chemistry.chemical_compound
medicine
Animals
Mice, Inbred BALB C
Tumor Necrosis Factor-alpha
Dendritic Cells
Dendritic cell
biology.organism_classification
Enterobacteriaceae
Mice, Inbred C57BL
Infectious Diseases
chemistry
Salmonella enterica
Microbial Immunity and Vaccines
Mutation
Parasitology
Tumor necrosis factor alpha
Nitric Oxide Synthase
Interleukin-1
Subjects
Details
- ISSN :
- 10985522 and 00199567
- Volume :
- 71
- Database :
- OpenAIRE
- Journal :
- Infection and Immunity
- Accession number :
- edsair.doi.dedup.....ab538d5e06c6ad4b4644e3292fe65d6a