Back to Search
Start Over
Detection of mouse tyrosinase with a monoclonal antibody MAT-1 against human tyrosinase
- Source :
- Pigment cell research. 9(6)
- Publication Year :
- 1996
-
Abstract
- In this study we explored the possible application of MAT-1, which has been established as a monoclonal antibody against human tyrosinase, for detection of mouse tyrosinase. The MAT-1 reacted with B16 mouse melanoma cells, but not with tyrosinase-negative NIH-3T3 mouse fibroblasts. In western blot analysis of the large granule fraction (LGF) of B16 cells, MAT-1 detected a single protein of 80 kDa, whose size was close to that of human tyrosinase detected with MAT-1 in extracts of human melanocytes. Furthermore, the 80 kDa band that was detected with MAT-1 in the LGF of B16 cells was also detected by DOPA reaction. In order to confirm that the protein detected with MAT-1 is tyrosinase, a transient expression assay was carried out. When mouse tyrosinase or mouse tyrosinase-related protein 1, which shares high homology with human tyrosinase, was transiently expressed in tyrosinase-negative K1735 mouse melanoma cells by cDNA transfection, MAT-1 reacted only with the cells expressing mouse tyrosinase. These results indicate that MAT-1 specifically reacts with mouse tyrosinase.
- Subjects :
- medicine.drug_class
Tyrosinase
Clinical Biochemistry
Blotting, Western
Plant Science
Chemical Fractionation
Monoclonal antibody
Transfection
Mice
Western blot
medicine
Tumor Cells, Cultured
Animals
Humans
Melanoma
medicine.diagnostic_test
Chemistry
Monophenol Monooxygenase
Antibodies, Monoclonal
Cell Biology
Mouse Melanoma
Molecular biology
Isoenzymes
Molecular Weight
Cdna transfection
Agronomy and Crop Science
High homology
Developmental Biology
Subjects
Details
- ISSN :
- 08935785
- Volume :
- 9
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Pigment cell research
- Accession number :
- edsair.doi.dedup.....ab4b50a96c7346da47f72ef18fad69c5