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Long non‐coding RNA LINC01225 promotes proliferation, invasion and migration of gastric cancer via Wnt/β‐catenin signalling pathway
- Source :
- Journal of Cellular and Molecular Medicine
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Emerging evidence has classified the aberrant expression of long non‐coding RNAs (lncRNAs) as a basic signature of various malignancies including gastric cancer (GC). LINC01225 has been shown to act as a hepatocellular carcinoma‐related gene, with its expression pattern and biological function not clarified in GC. Here, we verified that LINC01225 was up‐regulated in tumour tissues and plasma of GC. Analysis with clinicopathological information suggested that up‐regulation of LINC01225 was associated with advanced disease and poorer overall survival. Receiver operating characteristic (ROC) analysis showed that plasma LINC01225 had a moderate accuracy for diagnosis of GC. In addition, knockdown of LINC01225 led to retardation of cell proliferation, invasion and migration, and overexpression of LINC01225 showed the opposite effects. Mechanistic investigations showed that LINC01225 silencing inhibited epithelial‐mesenchymal transition (EMT) process and attenuated Wnt/β‐catenin signalling of GC. Furthermore, ectopic expression of Wnt1 or suppression of GSK‐3β abolished the si‐LINC01225‐mediated suppression against EMT, thereby promoting cell proliferation, invasion and migration of GC. In conclusion, LINC01225 promotes the progression of GC through Wnt/β‐catenin signalling pathway, and it may serve as a potential target or strategy for diagnosis or treatment of GC.
- Subjects :
- Male
0301 basic medicine
Epithelial-Mesenchymal Transition
Carcinogenesis
LINC01225
Wnt
03 medical and health sciences
0302 clinical medicine
epithelial‐mesenchymal transition (EMT)
Cell Movement
Stomach Neoplasms
Cell Line, Tumor
medicine
Animals
Humans
Gene silencing
Neoplasm Invasiveness
Wnt Signaling Pathway
Aged
Cell Proliferation
Neoplasm Staging
Mice, Inbred BALB C
Gene knockdown
Glycogen Synthase Kinase 3 beta
Chemistry
Cell growth
Wnt signaling pathway
Cancer
Original Articles
Cell Biology
Middle Aged
medicine.disease
Hedgehog signaling pathway
Long non-coding RNA
Up-Regulation
gastric cancer (GC)
Gene Expression Regulation, Neoplastic
030104 developmental biology
Gene Knockdown Techniques
030220 oncology & carcinogenesis
Cancer research
β‐catenin
Molecular Medicine
Original Article
Female
RNA, Long Noncoding
Ectopic expression
Subjects
Details
- ISSN :
- 15824934 and 15821838
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular and Molecular Medicine
- Accession number :
- edsair.doi.dedup.....ab44b030d4729cb21990dbf81419824d