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Caspase-8 sumoylation is associated with nuclear localization
- Source :
- Oncogene, Oncogene, Nature Publishing Group, 2005, 24 (20), pp.3268-73. ⟨10.1038/sj.onc.1208448⟩
- Publication Year :
- 2005
- Publisher :
- HAL CCSD, 2005.
-
Abstract
- The cysteine protease caspase-8 plays a pivotal role in the initiation of different apoptotic pathways and controls the maturation and differentiation of various cell types including neurons, fibroblasts and lymphocytes. Specific substrates of caspase-8 are present in both the cytoplasm and the nucleus, which may determine the ultimate biological effect of caspase-8. However, the mechanisms regulating the cellular localization of caspase-8 are still unknown. We show here that, in contrast to other caspases such as caspase-9 and -3, caspase-8 can be sumoylated at lysine 156. This sumoylation (i) is associated with the nuclear localization of caspase-8 and (ii) did not impair caspase-8 activation.
- Subjects :
- Cancer Research
Cytoplasm
SUMO protein
MESH: Neurons
Apoptosis
MESH: Amino Acid Sequence
MESH: Caspase 9
MESH: Caspase 8
Mass Spectrometry
Jurkat Cells
MESH: Protein Structure, Tertiary
0302 clinical medicine
MESH: Caspase 3
MESH: Jurkat Cells
Lymphocytes
Caspase
Cellular localization
Caspase-9
Neurons
0303 health sciences
Caspase 8
biology
Caspase 3
Caspase 9
3. Good health
Cell biology
Biochemistry
030220 oncology & carcinogenesis
Caspases
Electrophoresis, Polyacrylamide Gel
Anisomycin
Subcellular Fractions
MESH: Cell Nucleus
DNA, Complementary
MESH: Cell Line, Tumor
Blotting, Western
Molecular Sequence Data
SUMO-1 Protein
MESH: Anisomycin
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
MESH: Two-Hybrid System Techniques
03 medical and health sciences
Cell Line, Tumor
Two-Hybrid System Techniques
MESH: Gene Library
MESH: Nucleic Acid Synthesis Inhibitors
Genetics
Humans
Immunoprecipitation
MESH: Blotting, Western
MESH: Lysine
Amino Acid Sequence
Cysteine
Molecular Biology
030304 developmental biology
Gene Library
Nucleic Acid Synthesis Inhibitors
Cell Nucleus
MESH: Mass Spectrometry
MESH: Humans
MESH: Molecular Sequence Data
MESH: Caspases
MESH: Immunoprecipitation
Lysine
MESH: Cytoplasm
MESH: Apoptosis
MESH: SUMO-1 Protein
MESH: DNA, Complementary
Fibroblasts
MESH: Cysteine
Protein Structure, Tertiary
MESH: Fibroblasts
MESH: Subcellular Fractions
biology.protein
MESH: Lymphocytes
Nuclear localization sequence
MESH: Electrophoresis, Polyacrylamide Gel
Subjects
Details
- Language :
- English
- ISSN :
- 09509232 and 14765594
- Database :
- OpenAIRE
- Journal :
- Oncogene, Oncogene, Nature Publishing Group, 2005, 24 (20), pp.3268-73. ⟨10.1038/sj.onc.1208448⟩
- Accession number :
- edsair.doi.dedup.....ab42bae6a32ad047f1982687d0b9acbc
- Full Text :
- https://doi.org/10.1038/sj.onc.1208448⟩