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TCam-2 seminoma cells exposed to egg-derived microenvironment modify their shape, adhesive pattern and migratory behaviour. A molecular and morphometric analysis
- Source :
- PLoS ONE, PLoS ONE, Vol 8, Iss 10, p e76192 (2013)
- Publication Year :
- 2013
- Publisher :
- Public Library of Science, 2013.
-
Abstract
- Seminoma is one of the most common Testicular Germ Cell Tumours that originates during embryonic development due to an alteration of the local niche that in turn results in a delayed or blocked differentiation of Primordial Germ Cells. The block of differentiation is actually a common way to develop cancer disease as postulated by the "embryonic rest theory of cancer". In agreement with this theory different studies have demonstrated that embryonic cues display the capacity of reprogramming aggressive cancer cells towards a less aggressive phenotype. Herein we investigate the ability of a culture medium added with 10% egg albumen (EW, Egg White) to modulate seminoma cell phenotype and behaviour, by ensuring a proper set of morphogenetic signals. We chose to use the TCam-2 seminoma cell line that has been established as the only available cell line, obtained from a primary testicular seminoma. EW is able to: 1) modify TCam-2 cell spreading rate and cell-substrate adhesion without affecting proliferation and survival indexes; 2) modulate TCam-2 actin distribution pattern increasing cortical localization of actin filaments; 3) increase TCam-2 cell-cell junction capability; 4) decrease both chemo-sensitive and collective TCam-2 migratory behaviour. According to these observations morphometric fractal analysis revealed the ability of EW to increase Circularity and Solidity parameters and, consequently, to decrease Fractal dimension. Prompted by these observations we hypothesize that EW treatment could rescue, at least in part, the neoplastic-metastatic behaviour of seminoma cells.
- Subjects :
- Male
Genetics and Molecular Biology (all)
Pathology
Biochemistry
Cell Movement
Actins
Animals
Antigens, CD29
Cadherins
Cell Adhesion
Cell Line, Tumor
Cell Proliferation
Cell Shape
Cell Survival
Connexin 43
Egg Proteins
Egg White
Focal Adhesions
Humans
Intercellular Junctions
Seminoma
Testicular Neoplasms
Vinculin
beta Catenin
Agricultural and Biological Sciences (all)
Biochemistry, Genetics and Molecular Biology (all)
Medicine (all)
Cytoskeleton
Multidisciplinary
Tumor
Integrin beta1
Cell migration
Cell biology
Medicine
Reprogramming
Research Article
CD29
medicine.medical_specialty
Science
Biology
Cell Line
medicine
Antigens
Actin
Embryogenesis
medicine.disease
Embryonic stem cell
Cell culture
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, PLoS ONE, Vol 8, Iss 10, p e76192 (2013)
- Accession number :
- edsair.doi.dedup.....ab3f41c5de12f6a1d8a77dce07c736d0