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Poly(I:C) coated PLGA microparticles induce dendritic cell maturation
- Source :
- International Journal of Pharmaceutics. 365:61-68
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- Microparticles from poly(D,L-lactic-co-glycolic acid) [PLGA] are of steadily rising interest for the delivery of antigens to immune cells and the induction of a long-lasting immune response for vaccination or immunological tumor therapy. However, if the desired vaccine contains only weak antigens and fails to activate the antigen presenting cells (APC), the opposite effect, i.e., the induction of immunotolerance may be observed. Therefore, it was the aim of this study to show the ability of protein loaded PLGA microparticles to additionally carry a specific, surface-coated maturation signal to human dendritic cells (DC), i.e., the most potent APC. Polyinosine-polycytidylic acid [poly(I:C)], a ligand of Toll-like receptor (TLR) 3, was efficiently bound either in a single layer or a multilayer attempt to the surface of diethylaminoethyl dextran modified PLGA microparticles. These particles were effectively phagocytized by DC ex vivo and induced a maturation similar to that achieved with a cytokine cocktail or higher concentrations of soluble poly(I:C). In conclusion, the concept of surface coating of biodegradable microparticles with selected TLR ligands might successfully be used in DC-based cell therapies for cancer or in vaccination trials to induce DC maturation and specifically amplify the immunological response to encapsulated antigens.
- Subjects :
- medicine.medical_treatment
Pharmaceutical Science
02 engineering and technology
Ligands
03 medical and health sciences
chemistry.chemical_compound
Immune system
Phagocytosis
Polylactic Acid-Polyglycolic Acid Copolymer
Antigen
medicine
Humans
Lactic Acid
Antigen-presenting cell
Cells, Cultured
030304 developmental biology
0303 health sciences
Toll-like receptor
Chemistry
DEAE-Dextran
Dendritic Cells
Dendritic cell
021001 nanoscience & nanotechnology
Molecular biology
Microspheres
Toll-Like Receptor 3
3. Good health
Surface coating
PLGA
Poly I-C
0210 nano-technology
Adjuvant
Polyglycolic Acid
Subjects
Details
- ISSN :
- 03785173
- Volume :
- 365
- Database :
- OpenAIRE
- Journal :
- International Journal of Pharmaceutics
- Accession number :
- edsair.doi.dedup.....ab3222ea59828da0a5598170d4615f02