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Surface modification of glycidyl-containing poly(methyl methacrylate) microchips using surface-initiated atom-transfer radical polymerization
- Source :
- Analytical chemistry. 80(3)
- Publication Year :
- 2008
-
Abstract
- Fabrication of microfluidic systems from polymeric materials is attractive because of simplicity and low cost. Unfortunately, the surfaces of many polymeric materials can adsorb biological samples. Therefore, it is necessary to modify their surfaces before these polymeric materials can be used for separation and analysis. Oftentimes it is difficult to modify polymeric surfaces because of their resistance to chemical reaction. Recently, we introduced a surface-reactive acrylic polymer, poly(glycidyl methacrylate-co-methyl methacrylate) (PGMAMMA), which can be modified easily and is suitable for fabrication of microfluidic devices. Epoxy groups on the surface can be activated by air plasma treatment, hydrolysis, or aminolysis. In this work, the resulting hydroxyl or amino groups were reacted with 2-bromoisobutylryl bromide to introduce an initiator for surface-initiated atom-transfer radical polymerization (SI-ATRP). Polyethylene glycol (PEG) layers grown on the surface using this method were uniform, hydrophilic, stable, and resistant to protein adsorption. Contact angle measurement and X-ray photoelectron spectroscopy (XPS) were used to characterize activated polymer surfaces, initiator-bound surfaces, and PEG-grafted surfaces. We obtained excellent capillary electrophoresis (CE) separations of proteins and peptides with the PEG-modified microchips. A separation efficiency of 4.4 x 10(4) plates for a 3.5 cm long separation channel was obtained.
- Subjects :
- Time Factors
Free Radicals
Surface Properties
Radical polymerization
Polyethylene glycol
Methacrylate
Analytical Chemistry
Polyethylene Glycols
chemistry.chemical_compound
Lab-On-A-Chip Devices
Polymer chemistry
Microchip Analytical Procedures
Polymethyl Methacrylate
Amines
chemistry.chemical_classification
Atom-transfer radical-polymerization
Hydrolysis
Spectrum Analysis
technology, industry, and agriculture
Electrophoresis, Capillary
Polymer
Microfluidic Analytical Techniques
Poly(methyl methacrylate)
Hydrocarbons, Brominated
chemistry
Polymerization
visual_art
visual_art.visual_art_medium
Surface modification
Epoxy Compounds
Methacrylates
Subjects
Details
- ISSN :
- 00032700
- Volume :
- 80
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Analytical chemistry
- Accession number :
- edsair.doi.dedup.....ab0c8cd9e141515c313d45cacac8e0ae