Back to Search
Start Over
Modulation of visceral hypersensitivity by glial cell line-derived neurotrophic factor family receptoralpha;-3 in colorectal afferents
- Source :
- American journal of physiology. Gastrointestinal and liver physiology. 300(3)
- Publication Year :
- 2011
-
Abstract
- Irritable bowel syndrome is characterized by colorectal hypersensitivity and contributed to by sensitized mechanosensitive primary afferents and recruitment of mechanoinsensitive (silent) afferents. Neurotrophic factors are well known to orchestrate dynamic changes in the properties of sensory neurons. Although pain modulation by proteins in the glial cell line-derived neurotrophic factor (GDNF) family has been documented in various pathophysiological states, their role in colorectal hypersensitivity remains unexplored. Therefore, we investigated the involvement of the GDNF family receptor α-3 (GFRα3) signaling in visceral hypersensitivity by quantifying visceromotor responses (VMR) to colorectal distension before and after intracolonic treatment with 2,4,6-trinitrobenzene sulfonic acid (TNBS). Baseline responses to colorectal distension did not differ between C57BL/6 and GFRα3 knockout (KO) mice. Relative to intracolonic saline treatment, TNBS significantly enhanced the VMR to colorectal distension in C57BL/6 mice 2, 7, 10, and 14 days posttreatment, whereas TNBS-induced visceral hypersensitivity was significantly suppressed in GFRα3 KO mice. The proportion of GFRα3 immunopositive thoracolumbar and lumbosacral colorectal dorsal root ganglion neurons was significantly elevated 2 days after TNBS treatment. In single fiber recordings, responses to circumferential stretch of colorectal afferent endings in C57BL/6 mice were significantly increased (sensitized) after exposure to an inflammatory soup, whereas responses to stretch did not sensitize in GFRα3 KO mice. These findings suggest that enhanced GFRα3 signaling in visceral afferents may contribute to development of colorectal hypersensitivity.
- Subjects :
- medicine.medical_specialty
Pathology
Glial Cell Line-Derived Neurotrophic Factor Receptors
Time Factors
Physiology
Colon
medicine.medical_treatment
Nerve Tissue Proteins
Mechanotransduction, Cellular
Neuroregulation and Motility
Mice
Dorsal root ganglion
Neurotrophic factors
Physiology (medical)
Internal medicine
Ganglia, Spinal
medicine
Glial cell line-derived neurotrophic factor
Pressure
Animals
Neurons, Afferent
Receptor
Evoked Potentials
Irritable bowel syndrome
Mice, Knockout
Hepatology
biology
business.industry
Electromyography
Growth factor
Gastroenterology
Rectum
Visceral pain
medicine.disease
Colitis
Pathophysiology
Mice, Inbred C57BL
Disease Models, Animal
medicine.anatomical_structure
Endocrinology
Trinitrobenzenesulfonic Acid
Hyperalgesia
biology.protein
medicine.symptom
business
Subjects
Details
- ISSN :
- 15221547
- Volume :
- 300
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- American journal of physiology. Gastrointestinal and liver physiology
- Accession number :
- edsair.doi.dedup.....ab06dcfa1ecaf08ca2d2256610c9160d