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Role of Plasmodium berghei ookinete surface and oocyst capsule protein, a novel oocyst capsule-associated protein, in ookinete motility
- Source :
- Parasites & Vectors, Parasites & Vectors, Vol 14, Iss 1, Pp 1-12 (2021)
- Publication Year :
- 2021
- Publisher :
- Research Square Platform LLC, 2021.
-
Abstract
- Background Plasmodium sp., which causes malaria, must first develop in mosquitoes before being transmitted. Upon ingesting infected blood, gametes form in the mosquito lumen, followed by fertilization and differentiation of the resulting zygotes into motile ookinetes. Within 24 h of blood ingestion, these ookinetes traverse mosquito epithelial cells and lodge below the midgut basal lamina, where they differentiate into sessile oocysts that are protected by a capsule. Methods We identified an ookinete surface and oocyst capsule protein (OSCP) that is involved in ookinete motility as well as oocyst capsule formation. Results We found that knockout of OSCP in parasite decreases ookinete gliding motility and gradually reduces the number of oocysts. On day 15 after blood ingestion, the oocyst wall was significantly thinner. Moreover, adding anti-OSCP antibodies decreased the gliding speed of wild-type ookinetes in vitro. Adding anti-OSCP antibodies to an infected blood meal also resulted in decreased oocyst formation. Conclusion These findings may be useful for the development of a transmission-blocking tool for malaria. Graphical abstract
- Subjects :
- 0301 basic medicine
Male
Gliding motility
Plasmodium berghei
030106 microbiology
Protozoan Proteins
Motility
Antibodies, Protozoan
Infectious and parasitic diseases
RC109-216
Mosquito Vectors
Plasmodium
03 medical and health sciences
Mice
Oocyst
Microscopy, Electron, Transmission
parasitic diseases
medicine
Animals
Transmission block
Ookinete
Mice, Inbred BALB C
biology
Research
fungi
Oocysts
Midgut
biology.organism_classification
Blood meal
Cell biology
Malaria
030104 developmental biology
Infectious Diseases
medicine.anatomical_structure
Culicidae
biology.protein
Knock out parasite
Parasitology
Basal lamina
Female
Protein A
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Parasites & Vectors, Parasites & Vectors, Vol 14, Iss 1, Pp 1-12 (2021)
- Accession number :
- edsair.doi.dedup.....aaeac822e7d7b430fa1ee074e3064934