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The endoderm of the mouse embryo arises by dynamic widespread intercalation of embryonic and extraembryonic lineages
- Source :
- Developmental cell. 15(4)
- Publication Year :
- 2008
-
Abstract
- SummaryThe cell movements underlying the morphogenesis of the embryonic endoderm, the tissue that will give rise to the respiratory and digestive tracts, are complex and not well understood. Using live imaging combined with genetic labeling, we investigated the cell behaviors and fate of the visceral endoderm during gut endoderm formation in the mouse gastrula. Contrary to the prevailing view, our data reveal no mass displacement of visceral endoderm to extraembryonic regions concomitant with the emergence of epiblast-derived definitive endoderm. Instead, we observed dispersal of the visceral endoderm epithelium and extensive mixing between cells of visceral endoderm and epiblast origin. Visceral endoderm cells remained associated with the epiblast and were incorporated into the early gut tube. Our findings suggest that the segregation of extraembryonic and embryonic tissues within the mammalian embryo is not as strict as believed and that a lineage previously defined as exclusively extraembryonic contributes cells to the embryo.
- Subjects :
- animal structures
Green Fluorescent Proteins
Respiratory System
Extraembryonic Membranes
DEVBIO
Mice, Transgenic
Histogenesis
Biology
Models, Biological
Article
General Biochemistry, Genetics and Molecular Biology
Mice
Endoderm formation
Embryonic disc
medicine
Image Processing, Computer-Assisted
Morphogenesis
Animals
Cell Lineage
RNA, Messenger
Transgenes
Molecular Biology
In Situ Hybridization
Endoderm
Diploblasty
Gene Expression Regulation, Developmental
Cell Biology
Gastrula
Embryo, Mammalian
Immunohistochemistry
Cell biology
Gastrulation
Gastrointestinal Tract
medicine.anatomical_structure
Epiblast
embryonic structures
Developmental Biology
Definitive endoderm
Subjects
Details
- ISSN :
- 18781551
- Volume :
- 15
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Developmental cell
- Accession number :
- edsair.doi.dedup.....aae16bd8420d4a92675935d3df6ff9e0