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Design of Ratiometric Emission Probe with Visible Light Excitation for Determination of Ca2+ in Living Cells
- Source :
- Analytical Chemistry. 86:8025-8030
- Publication Year :
- 2014
- Publisher :
- American Chemical Society (ACS), 2014.
-
Abstract
- An organic salt as a fluorescent probe based on intramolecular charge transfer for Ca(2+) determination is developed. Ca(2+) can be detected by ratiometric emission at 490 and 594 nm with an excitation wavelength of 405 nm. This probe is highly selective for Ca(2+) over other divalent metal cations and displays a large Stokes shift of 189 nm that can avoid interference of the excitation light beam and autofluorescence of biological samples. The dissociation constant for Ca(2+) is 2.25 ± 0.47 μM and pertinent to Ca(2+) detection in cellular resting and dynamic states. The probe demonstrates its application in monitoring Ca(2+) in living cells under confocal microscopic imaging.
- Subjects :
- Oxadiazoles
Microscopy, Confocal
Light
Cations, Divalent
Chemistry
Confocal
Optical Imaging
Analytical chemistry
Photochemistry
Fluorescence
Analytical Chemistry
Dissociation constant
symbols.namesake
Autofluorescence
Spectrometry, Fluorescence
Stokes shift
Human Umbilical Vein Endothelial Cells
symbols
Humans
Light beam
Calcium
Egtazic Acid
Excitation
Fluorescent Dyes
Visible spectrum
Subjects
Details
- ISSN :
- 15206882 and 00032700
- Volume :
- 86
- Database :
- OpenAIRE
- Journal :
- Analytical Chemistry
- Accession number :
- edsair.doi.dedup.....aada32ef885a73fdc842b6a592a7280d
- Full Text :
- https://doi.org/10.1021/ac5022002