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Design of Ratiometric Emission Probe with Visible Light Excitation for Determination of Ca2+ in Living Cells

Authors :
Li Fan
Chuan Dong
Qin Hu
Martin M. F. Choi
Shaomin Shuang
Xiaoze Ren
Huizhi Du
Wei Bian
Qiaoling Liu
Source :
Analytical Chemistry. 86:8025-8030
Publication Year :
2014
Publisher :
American Chemical Society (ACS), 2014.

Abstract

An organic salt as a fluorescent probe based on intramolecular charge transfer for Ca(2+) determination is developed. Ca(2+) can be detected by ratiometric emission at 490 and 594 nm with an excitation wavelength of 405 nm. This probe is highly selective for Ca(2+) over other divalent metal cations and displays a large Stokes shift of 189 nm that can avoid interference of the excitation light beam and autofluorescence of biological samples. The dissociation constant for Ca(2+) is 2.25 ± 0.47 μM and pertinent to Ca(2+) detection in cellular resting and dynamic states. The probe demonstrates its application in monitoring Ca(2+) in living cells under confocal microscopic imaging.

Details

ISSN :
15206882 and 00032700
Volume :
86
Database :
OpenAIRE
Journal :
Analytical Chemistry
Accession number :
edsair.doi.dedup.....aada32ef885a73fdc842b6a592a7280d
Full Text :
https://doi.org/10.1021/ac5022002