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Ionophores at work: Exploring the interaction of guanosine-based amphiphiles with phospholipid membranes
- Source :
- Biochimica et biophysica acta / Biomembranes 1859(12), 2392-2401 (2017). doi:10.1016/j.bbamem.2017.09.007
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- An amphiphilic derivative of guanosine, carrying a myristoyl group at the 5'-position and two methoxy(triethylene glycol) appendages at the 2' and 3'-positions (1), endowed with high ionophoric activity, has been here studied in its interaction mode with a model lipid membrane along with its 5'-spin-labelled analogue 2, bearing the 5-doxyl-stearic in lieu of the myristic residue. Electron spin resonance spectra, carried out on the spin-labelled nucleolipid 2 in mixture with a DOPC/DOPG phospholipid bilayer, on one side, and on spin-labelled lipids mixed with 1, on the other, integrated with dynamic light scattering and neutron reflectivity measurements, allowed getting an in-depth picture of the effect of the ionophores on membrane structure, relevant to clarify the ion transport mechanism through lipid bilayers. Particularly, dehydration of lipid headgroups and lowering of both the local polarity and acyl chains order across the bilayer, due to the insertion of the oligo(ethylene glycol) chains in the bilayer hydrophobic core, have been found to be the main effects of the amphiphilic guanosines interaction with the membrane. These results furnish directions to rationally implement future ionophores design.
- Subjects :
- Guanosine derivative
Light
Stereochemistry
Lipid Bilayers
Biophysics
Phospholipid
02 engineering and technology
010402 general chemistry
01 natural sciences
Biochemistry
Polyethylene Glycols
chemistry.chemical_compound
Biomembrane
Electron spin resonance
ddc:570
Amphiphile
Scattering, Radiation
Lipid bilayer
Guanosine
Ionophores
Neutron reflectivity
Chemistry
Bilayer
Electron Spin Resonance Spectroscopy
Membrane structure
Phosphatidylglycerols
Biological membrane
Cell Biology
021001 nanoscience & nanotechnology
0104 chemical sciences
Kinetics
Crystallography
Membrane
Synthetic ionophore
Biophysic
Drug Design
Phosphatidylcholines
Spin Labels
lipids (amino acids, peptides, and proteins)
0210 nano-technology
Hydrophobic and Hydrophilic Interactions
Ethylene glycol
Subjects
Details
- ISSN :
- 00052736
- Volume :
- 1859
- Database :
- OpenAIRE
- Journal :
- Biochimica et Biophysica Acta (BBA) - Biomembranes
- Accession number :
- edsair.doi.dedup.....aad0f7f069636fac6a3e5ac291c23d1c