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Carotid Plaques From Symptomatic Patients Are Characterized by Local Increase in Xanthine Oxidase Expression
- Source :
- Stroke. 52:2792-2801
- Publication Year :
- 2021
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2021.
-
Abstract
- Background and Purpose: XO (xanthine oxidase) is a key enzyme of uric acid metabolism and is thought to contribute to oxidative pathways that promote atherosclerotic plaque progression, yet its role in plaque destabilization is not well elucidated. We hypothesized that XO is expressed in carotid plaque from symptomatic patients in association with cardiovascular risk factors. Methods: Patients were stratified by symptoms, defined as presentation with an ipsilateral cerebral ischemic event. Carotid atherosclerotic plaques were obtained from 44 patients with symptomatic plaque and 44 patients without ischemic cerebral events. Protein expression of XO was evaluated by immunohistochemical staining and the percentage of cells expressing XO and CD68 (macrophage marker) compared between the groups. Biochemical and demographic cardiometabolic risk factors of study participants also were measured. Results: Carotid atherosclerotic plaques from symptomatic patients were associated with significantly higher XO expression versus asymptomatic plaque (median [interquartile range]: 1.24 [2.09] versus 0.16 [0.34]; P P P =0.010, r =−0.30) and directly with circulating uric acid levels ( P r =0.45). The average percentage of macrophages that expressed XO was significantly higher in symptomatic versus asymptomatic plaques (median [interquartile range]: 93.37% [25] versus 46.15% [21], respectively; P Conclusions: XO overexpression in macrophages is associated with increased serum uric acid and low high-density lipoproteins cholesterol levels and may potentially have a mechanistic role in carotid plaque destabilization. The current study supports a potential role for uric acid synthesis pathway as a target for management of carotid atherosclerosis in humans.
- Subjects :
- Carotid Artery Diseases
Male
Xanthine Oxidase
Pathology
medicine.medical_specialty
030204 cardiovascular system & hematology
Asymptomatic
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Interquartile range
medicine
Humans
Macrophage
Carotid Stenosis
Xanthine oxidase
Aged
030304 developmental biology
Advanced and Specialized Nursing
Endarterectomy, Carotid
0303 health sciences
CD68
Cholesterol
business.industry
Macrophages
Plaque, Atherosclerotic
Carotid Arteries
chemistry
Uric acid
Immunohistochemistry
Female
Neurology (clinical)
medicine.symptom
Cardiology and Cardiovascular Medicine
business
Biomarkers
Subjects
Details
- ISSN :
- 15244628 and 00392499
- Volume :
- 52
- Database :
- OpenAIRE
- Journal :
- Stroke
- Accession number :
- edsair.doi.dedup.....aacde678489dde494ef62ab750fb8a7b