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Rifampicin-fosfomycin coating for cementless endoprostheses: antimicrobial effects against methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA)
- Source :
- Acta biomaterialia. 10(10)
- Publication Year :
- 2014
-
Abstract
- New strategies to decrease infection rates in cementless arthroplasty are needed, especially in the context of the growing incidence of methicillin-resistant Staphylococcus aureus (MRSA) infections. The purpose of this study was to investigate the antimicrobial activity of a rifampicin–fosfomycin coating against methicillin-sensitive Staphylococcus aureus (MSSA) and MRSA in a rabbit infection prophylaxis model. Uncoated or rifampicin–fosfomycin-coated K-wires were inserted into the intramedullary canal of the tibia in rabbits and contaminated with an inoculation dose of 10 5 or 10 6 colony-forming units of MSSA EDCC 5055 in study 1 and MRSA T6625930 in study 2, respectively. After 28 days the animals were killed and clinical, histological and microbiological assessment, including pulse-field gel electrophoresis, was conducted. Positive culture growth in agar plate testing and/or clinical signs and/or histological signs were defined positive for infection. Statistical evaluation was performed using Fisher’s exact test. Both studies showed a statistically significant reduction of infection rates for rifampicin–fosfomycin-coated implants compared to uncoated K-wires ( P = 0.015). In both studies none of the 12 animals that were treated with a rifampicin–fosfomycin-coated implant showed clinical signs of infection or a positive agar plate testing result. In both studies, one animal of the coating group showed the presence of sporadic bacteria with concomitant inflammatory signs in histology. The control groups in both studies exhibited an infection rate of 100% with clear clinical signs of infection and positive culture growth in all animals. In summary, the rifampicin–fosfomycin-coating showed excellent antimicrobial activity against both MSSA and MRSA, and therefore warrants further clinical testing.
- Subjects :
- Methicillin-Resistant Staphylococcus aureus
Biomedical Engineering
Context (language use)
Fosfomycin
medicine.disease_cause
Biochemistry
Microbiology
Biomaterials
Agar plate
Coated Materials, Biocompatible
Materials Testing
medicine
Animals
Molecular Biology
Antibiotics, Antitubercular
business.industry
Histology
General Medicine
Prostheses and Implants
biochemical phenomena, metabolism, and nutrition
Staphylococcal Infections
bacterial infections and mycoses
Antimicrobial
Methicillin-resistant Staphylococcus aureus
Staphylococcus aureus
Rabbits
Rifampin
business
Rifampicin
Biotechnology
medicine.drug
Bone Wires
Subjects
Details
- ISSN :
- 18787568
- Volume :
- 10
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Acta biomaterialia
- Accession number :
- edsair.doi.dedup.....aacade04d9d02ca350f3f9c1b3daa9fd