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Effect of different long-term fertilizer managements on soil nitrogen fixing bacteria community in a double-cropping rice paddy field of southern China
- Source :
- PLoS ONE, Vol 16, Iss 9, p e0256754 (2021), PLoS ONE
- Publication Year :
- 2021
- Publisher :
- Public Library of Science (PLoS), 2021.
-
Abstract
- Soil microorganism plays an important role in nitrogen (N) fixation process of paddy field, but the related information about how soil microorganism that drive N fixation process response to change of soil phy-chemical characteristics under the double-cropping rice (Oryza sativa L.) paddy field in southern of China is need to further study. Therefore, the impacts of 34-years different long-term fertilization system on soil N-fixing bacteria community under the double-cropping rice paddy field in southern of China were investigated by taken chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) method in this paper. The field experiment were set up four different fertilizer treatments: chemical fertilizer alone (MF), rice straw and chemical fertilizer (RF), 30% organic manure and 70% chemical fertilizer (OM), and unfertilized as a control (CK). This results showed that compared with CK treatment, the diversity index of cbbLR and nifH genes with OM and RF treatments were significantly increased (pcbbLR gene with OM, RF and MF treatments were increased by 23.94, 12.19 and 6.70×107 copies g-1 compared to CK treatment, respectively. Compared with CK treatment, the abundance of nifH gene with OM, RF and MF treatments were increased by 23.90, 8.82 and 5.40×109 copies g-1, respectively. This results indicated that compared with CK treatment, the soil autotrophic azotobacter and nitrogenase activities with OM and RF treatments were also significantly increased (p
- Subjects :
- Crop residue
Artificial Gene Amplification and Extension
Soil Chemistry
Multiple cropping
Polymerase Chain Reaction
Soil
Agricultural Soil Science
Soil Microbiology
Multidisciplinary
Ecology
Eukaryota
food and beverages
Soil chemistry
Agriculture
Plants
Crop Production
Chemistry
Community Ecology
Experimental Organism Systems
Physical Sciences
Medicine
Fertilizer
Agrochemicals
Research Article
Nitrogen-Fixing Bacteria
China
Nitrogen
Science
Soil Science
Crops
Biology
engineering.material
Research and Analysis Methods
Plant and Algal Models
Environmental Chemistry
Soil ecology
Grasses
Fertilizers
Molecular Biology Techniques
Community Structure
Molecular Biology
Azotobacter
Oryza sativa
Bacteria
Ecology and Environmental Sciences
Organisms
Biology and Life Sciences
Oryza
biology.organism_classification
Agronomy
Earth Sciences
Animal Studies
engineering
Paddy field
Rice
Crop Science
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....aac4c5b079d9fa88638f46642cafc547