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Genome-wide hypomethylation of LINE-1 and Alu retroelements in cell-free DNA of blood is an epigenetic biomarker of human aging
- Source :
- Saudi Journal of Biological Sciences, Vol 25, Iss 6, Pp 1220-1226 (2018)
- Publication Year :
- 2017
-
Abstract
- Aging associated DNA hypomethylation of LINE-1 and Alu retroelements may be a crucial determinant of loss of genomic integrity, deterioration and cancer. In peripheral blood LINE-1 hypomethylation has been reported to increase during aging, but other studies did not observe significant changes. We hypothesized that these apparently inconsistent reports might relate to differences between cellular and cell-free DNA. Using the technique of idiolocal normalization of real-time methylation-specific PCR (IDLN-MSP) for genetic imbalanced DNA specimens we obtained evidence that LINE-1 hypomethylation in cell-free DNA, but not cellular DNA from peripheral blood is an epigenetic biomarker for human aging. Furthermore, hypomethylation of cell-free DNA is more extensive in smokers, suggesting that it might be used as a surrogate marker for monitoring the improvement of smoking-induced adverse effects after cancelling smoking. Keywords: Epigenetics, DNA methylation, Cell-free DNA, Retroelements, LINE-1
- Subjects :
- 0301 basic medicine
Cancer
Biology
medicine.disease
Genome
Article
03 medical and health sciences
Biomarker
chemistry.chemical_compound
030104 developmental biology
lcsh:Biology (General)
Cell-free fetal DNA
chemistry
DNA methylation
medicine
Cancer research
Epigenetics
General Agricultural and Biological Sciences
lcsh:QH301-705.5
DNA
DNA hypomethylation
Subjects
Details
- ISSN :
- 1319562X
- Volume :
- 25
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Saudi journal of biological sciences
- Accession number :
- edsair.doi.dedup.....aaa685dbc7c4633176f3233d9f659424