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Efficacy of Amphotericin B in Corneal Preservation Media After Extended Frozen Storage
- Source :
- Cornea. 39:1274-1277
- Publication Year :
- 2020
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2020.
-
Abstract
- Purpose To investigate the antimycotic activity of amphotericin B deoxycholate that has been previously frozen for 28 days before supplementation of Optisol-GS. Methods Triplicate Optisol-GS samples were inoculated with 10 colony-forming units (CFU) of Candida albicans. Each set of triplicate cultures was supplemented with 2.5 μg/mL of amphotericin B that was either freshly resuspended and never frozen, frozen overnight at -20°C and thawed, or frozen at -20°C for 4 weeks and thawed. The cultures were stored at 4°C, with aliquots taken at 0, 6, 24, and 72 hours for quantification. The efficacy of each preparation of amphotericin B in reducing C. albicans growth was assessed at these time points. Results Six hours after antifungal supplementation, there was a 1.33 log10 CFU reduction with freshly resuspended amphotericin B, compared with a 1.31 log10 reduction with amphotericin B that was frozen overnight (P = 0.20) and a 1.18 log10 reduction with amphotericin B that was frozen for 4 weeks (P = 0.05). After 72 hours, there was a 2.72 log10 CFU reduction with freshly resuspended amphotericin B, a 2.64 log10 CFU reduction with amphotericin B that was frozen overnight (P = 0.45), and a 2.18 log10 CFU reduction with amphotericin B that was frozen for 4 weeks (P = 0.05). Conclusions Previously frozen amphotericin B remains highly effective against C. albicans. Optisol-GS supplemented with 2.5 μg/mL amphotericin B that was frozen for 4 weeks at -20°C resulted in >90% CFU reduction by 6 hours and >99% reduction by 72 hours.
- Subjects :
- Antifungal
Antifungal Agents
medicine.drug_class
Organ Preservation Solutions
Microbial Sensitivity Tests
Complex Mixtures
Culture Media, Serum-Free
Microbiology
Cornea
Amphotericin B
Amphotericin B deoxycholate
Candida albicans
medicine
Humans
Log10 reduction
Cryopreservation
biology
Chemistry
Chondroitin Sulfates
Dextrans
Organ Preservation
biology.organism_classification
Corpus albicans
Drug Combinations
Ophthalmology
Treatment Outcome
Frozen storage
Gentamicins
Deoxycholic Acid
medicine.drug
Subjects
Details
- ISSN :
- 02773740
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- Cornea
- Accession number :
- edsair.doi.dedup.....aa9a5cbf7a88cf1f6cd2d0b5bb895fd2