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Inorganic arsenite inhibits IgE receptor-mediated degranulation of mast cells
- Source :
- Journal of applied toxicology : JAT. 31(3)
- Publication Year :
- 2009
-
Abstract
- Millions of people worldwide are exposed to arsenic (As), a toxicant which increases the risk of various cancers, cardiovascular disease and several other health problems. Arsenic is a potent endocrine disruptor, including of the estrogen receptor. It was recently shown that environmental estrogen-receptor disruptors can affect the signaling of mast cells, which are important players in parasite defense, asthma and allergy. Antigen (Ag) or allergen crosslinking of IgE-bound receptors on mast cells leads to signaling, culminating in degranulation, the release of histamine and other mediators. Because As is an endocrine disruptor and because endocrine disruptors have been found to affect degranulation, here we have tested whether sodium arsenite affects degranulation. Using the rat basophilic leukemia (RBL) mast cell model, we have measured degranulation in a fluorescence assay. Arsenic alone had no effect on basal levels of degranulation. However, As strongly inhibited Ag-stimulated degranulation at environmentally relevant concentrations, in a manner that is very dependent on concentrations of both As and Ag. The concentrations of As effective at inhibiting degranulation were not cytotoxic. This inhibition may be a mechanism underlying the traditional Chinese medicinal use of As to treat asthma. These data indicate that As may inhibit the ability of humans to fight off parasitic disease.
- Subjects :
- Allergy
Sodium arsenite
Arsenites
Cell Survival
Endocrine Disruptors
Toxicology
Immunoglobulin E
Cell Degranulation
chemistry.chemical_compound
Cell Line, Tumor
medicine
Animals
Mast Cells
Receptor
biology
Dose-Response Relationship, Drug
L-Lactate Dehydrogenase
Receptors, IgE
Degranulation
Antibodies, Monoclonal
Trypan Blue
Allergens
Mast cell
medicine.disease
Sodium Compounds
Rats
medicine.anatomical_structure
Endocrine disruptor
chemistry
Immunology
biology.protein
Drug Therapy, Combination
Drug Antagonism
Histamine
Subjects
Details
- ISSN :
- 10991263
- Volume :
- 31
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of applied toxicology : JAT
- Accession number :
- edsair.doi.dedup.....aa84db42e5ce0ddb687ca5deac3faf1f