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Development and validation of a HPLC method for determination of levonorgestrel and quinestrol in rat plasma
- Source :
- Biomedical Chromatography. 24:706-710
- Publication Year :
- 2009
- Publisher :
- Wiley, 2009.
-
Abstract
- Levonorgestrel and quinestrol, commonly known as EP-1, has long been used in the control of wild rodents. Up to the present time, however, no method for simultaneous quantification of levonorgestrel and quinestrol in rat plasma has been reported. In the present study, a sensitive reverse-phase high-performance liquid chromatography with ultraviolet detection (RP-HPLC-UV) method for quantification of levonorgestrel and quinestrol in rat plasma has been developed. It uses a Kromasil ODS C(18) column and acetonitrile-0.1% formic acid (85 : 15, v/v) mobile phase at ambient temperature. The plasma sample was prepared by hexane-isoamyl alcohol extraction (90 : 10, v/v). The flow rate and detection wavelength were 1.0 mL/min and 230 nm. The correlation coefficients were greater than 0.9995 within 0.08-50 microg/mL for levonorgestrel and 0.12-50 microg/mL for quinestrol, and the limits of detection were 0.02 and 0.05 microg/mL for levonorgestrel and quinestrol, respectively. Average recovery ranged from 92.5 to 96.3% and inter-day RSDs were less than 7.56%. This method can be applied to the further pharmacokinetic study of levonorgestrel and quinestrol in rat plasma.
- Subjects :
- endocrine system
Formic acid
Clinical Biochemistry
Quinestrol
Levonorgestrel
Biochemistry
Analytical Chemistry
chemistry.chemical_compound
Pharmacokinetics
Limit of Detection
Drug Discovery
medicine
Animals
Hplc method
Molecular Biology
Chromatography, High Pressure Liquid
Pharmacology
Detection limit
Chromatography
Plasma samples
General Medicine
Plasma
Rats
chemistry
Contraceptives, Oral
medicine.drug
Subjects
Details
- ISSN :
- 10990801 and 02693879
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Biomedical Chromatography
- Accession number :
- edsair.doi.dedup.....aa6f65fe62368b59fa9b2b692c07e683
- Full Text :
- https://doi.org/10.1002/bmc.1350