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Diadinoxanthin de‐epoxidation as important factor in the short‐term stabilization of diatom photosynthetic membranes exposed to different temperatures
- Source :
- Plant, Cell & Environment. 42:1270-1286
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- The importance of diadinoxanthin (Ddx) de-epoxidation in the short-term modulation of the temperature effect on photosynthetic membranes of the diatom Phaeodactylum tricornutum was demonstrated by electron paramagnetic resonance (EPR), Laurdan fluorescence spectroscopy, and high-performance liquid chromatography. The 5-SASL spin probe employed for the EPR measurements and Laurdan provided information about the membrane area close to the polar head groups of the membrane lipids, whereas with the 16-SASL spin probe, the hydrophobic core, where the fatty acid residues are located, was probed. The obtained results indicate that Ddx de-epoxidation induces a two component mechanism in the short-term regulation of the membrane fluidity of diatom thylakoids during changing temperatures. One component has been termed the "dynamic effect" and the second the "stable effect" of Ddx de-epoxidation. The "dynamic effect" includes changes of the membrane during the time course of de-epoxidation whereas the "stable effect" is based on the rigidifying properties of Dtx. The combination of both effects results in a temporary increase of the rigidity of both peripheral and internal parts of the membrane whereas the persistent increase of the rigidity of the hydrophobic core of the membrane is solely based on the "stable effect."
- Subjects :
- 0106 biological sciences
0301 basic medicine
Physiology
Membrane lipids
Plant Science
Xanthophylls
Thylakoids
01 natural sciences
Spin probe
03 medical and health sciences
chemistry.chemical_compound
Membrane fluidity
Phaeodactylum tricornutum
Photosynthesis
Chromatography, High Pressure Liquid
Diatoms
biology
Chlorophyll A
Electron Spin Resonance Spectroscopy
Temperature
Diadinoxanthin
biology.organism_classification
Spectrometry, Fluorescence
030104 developmental biology
Membrane
chemistry
Thylakoid
Biophysics
Epoxy Compounds
Laurdan
010606 plant biology & botany
Subjects
Details
- ISSN :
- 13653040 and 01407791
- Volume :
- 42
- Database :
- OpenAIRE
- Journal :
- Plant, Cell & Environment
- Accession number :
- edsair.doi.dedup.....aa5723638a6c8f6a7c57778b8442e557