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Formation of post-confluence structure in human parotid gland acinar cells on PLGA through regulation of E-cadherin
- Source :
- Biomaterials. 33(2)
- Publication Year :
- 2011
-
Abstract
- As a potential solution for patients to retrieve their lost salivary gland functions, tissue engineering of an auto-secretory device is profoundly needed. Under serum-free environment, primary human parotid gland acinar (PGAC) cells can be obtained. After reaching confluence, PGAC cells spontaneously form three-dimension (3D) cell aggregations, termed post-confluence structure (PCS), and change their behaviors. Poly (lactic-co-glycolic acid) (PLGA) has been widely used in the field of biomedical applications because of its biodegradable properties for desired functions. Nonetheless, the role of PLGA in facilitating PGAC cells to form PCS has seldom been explored to recover epithelial characteristics. In this study, PGAC cells were found to have a greater tendency to form PCS on PLGA than on tissue culture polystyrene (TCPS). By tracing cell migration paths and modulating E-cadherin activity with specific inhibitor or antibody, we demonstrated that the static force of homophilic interaction on surfaces of individual cells, but not the dynamics of cell migration, played a more important role in PCS formation. Thus, PLGA was successfully confirmed to support PGAC cells to form more PCS through the effects on enhancing E-cadherin expression, which is associated with FAK/ILK/Snail expression in PGAC cells. This result indicates that selective appropriate biomaterials may be potentially useful in generating 3D PCS on two-dimension (2D) substrate without fabricating a complex 3D scaffold.
- Subjects :
- Materials science
Cellular differentiation
Blotting, Western
Biophysics
Bioengineering
Biocompatible Materials
Acinar Cells
Biomaterials
chemistry.chemical_compound
Tissue engineering
Downregulation and upregulation
Polylactic Acid-Polyglycolic Acid Copolymer
Cell Movement
Cell Adhesion
Humans
Parotid Gland
Lactic Acid
Cell adhesion
Cells, Cultured
Tissue Engineering
Tissue Scaffolds
Cadherin
Reverse Transcriptase Polymerase Chain Reaction
Cell migration
Cell Differentiation
Cadherins
Cell biology
Up-Regulation
PLGA
chemistry
Mechanics of Materials
Ceramics and Composites
Microscopy, Electron, Scanning
Signal transduction
Polyglycolic Acid
Biomedical engineering
Signal Transduction
Subjects
Details
- ISSN :
- 18785905
- Volume :
- 33
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Biomaterials
- Accession number :
- edsair.doi.dedup.....aa5630bba40f178a8dcb14fe069877e7