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Tissue Responses and Wound Healing following Laser Scleral Microporation for Presbyopia Therapy
- Source :
- Translational Vision Science & Technology
- Publication Year :
- 2020
- Publisher :
- Association for Research in Vision and Ophthalmology (ARVO), 2020.
-
Abstract
- Purpose To investigate the postoperative inflammatory and wound-healing responses after laser scleral microporation for presbyopia. Methods Thirty porcine eyes were used for the optimization of laser intensities first. Six monkeys (12 eyes) received scleral microporation with an erbium yttrium aluminum garnet (Er:YAG) laser, and half of the eyes received concurrent subconjunctival collagen gel to modulate wound-healing response. The intraocular pressure (IOP) and the laser ablation depth were evaluated. The animals were euthanized at 1, 6, and 9 months postoperatively. The limbal areas and scleras were harvested for histologic analysis and immunofluorescence of markers for inflammation (CD11b and CD45), wound healing (CD90, tenascin-C, fibronectin, and HSP47), wound contraction (α-smooth muscle actin [α-SMA]), vascular response (CD31), nerve injury (GAP43), and limbal stem cells (P63 and telomerase). Results In the nonhuman primate study, there was a significant reduction in IOP after the procedure. Overall, the ablation depth was 76.6% to 81.2% at 1 month and slightly decreased to 71.5% to 72.7% at 9 months. Coagulative necrosis around the micropores, as well as expression of CD11b, CD45, tenascin, fibronectin, HSP47, and GAP43, was distinct at 1 month but subsided with time. Collagen gel treatment significantly suppressed the upregulation of CD11b, CD45, fibronectin, and tenascin-C. The expression of CD90, α-SMA, and CD31 was minimal in all eyes. Conclusions The study demonstrated the course of inflammatory and wound-healing responses following laser scleral microporation. The tissue responses were small and self-limited, resolved with time, and were suppressed by concurrent collagen treatment. It provides a useful understanding of this new procedure. Translational relevance The results would be helpful in the laser parameter modification to improve the long-term treatment stability.
- Subjects :
- 0301 basic medicine
Intraocular pressure
medicine.medical_specialty
genetic structures
Swine
medicine.medical_treatment
Biomedical Engineering
Tenascin
Lasers, Solid-State
Article
03 medical and health sciences
0302 clinical medicine
Ophthalmology
medicine
Animals
Wound Healing
biology
business.industry
Nerve injury
Ablation
eye diseases
laser
Sclera
Fibronectin
030104 developmental biology
medicine.anatomical_structure
Coagulative necrosis
030221 ophthalmology & optometry
biology.protein
presbyopia
Laser Therapy
sense organs
medicine.symptom
scleral wound healing
Wound healing
business
Subjects
Details
- ISSN :
- 21642591
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Translational Vision Science & Technology
- Accession number :
- edsair.doi.dedup.....aa4a3c8f04e96d64db6eb2b355d30fbc
- Full Text :
- https://doi.org/10.1167/tvst.9.4.6