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Effect of cryoprotectants on the survival of cascudo preto (Rhinelepis aspera) embryos stored at –8°C
- Source :
- Zygote. 22:58-63
- Publication Year :
- 2011
- Publisher :
- Cambridge University Press (CUP), 2011.
-
Abstract
- SummaryCryopreservation of germplasm provides a promising method to preserve fish genetic material, which is of great importance in preservation of species diversity, aquaculture, and management of fish models used in biomedical research. In the present study, cryopreservation of Rhinelepis aspera embryos, a Brazilian endangered species, was studied for the first time using a short-term cooling protocol. Embryos at blastoporous closing stage were selected, placed in 6-ml glass vials and stored at −8°C for 6 h in 10 different cryoprotectant solutions: S1 (17.1% sucrose + 9% methanol); S2 (17.1% sucrose + 9% DMSO); S3 (8.5% sucrose + 8.5% glucose + 9% methanol); S4 (8.5% sucrose + 8.5% glucose + 9% DMSO); S5 (17.1% sucrose + 9% ethylene glycol); S6 (8.5% sucrose + 8.5% glucose + 9% ethylene glycol); S7 (17.1% sucrose + 4.5% methanol + 4.5% DMSO); S8 (17.1% sucrose + 4.5% methanol + 4.5% ethylene glycol); S9 (17.1% sucrose + 4.5% DMSO + 4.5% ethylene glycol); and S10 (100% water). Embryo viability was assessed by hatching rate, counting live larvae and number of failed eggs under a stereomicroscope. The results showed that only the cryoprotectant solutions that contained methanol associated to sucrose (S1, S7 and S8) provided partial protection of Rhinelepis aspera embryos from cold damage (over 50% hatching rate in S1), while the use of DMSO and ethylene glycol, isolated or in combination, resulted in no hatching rate. Further studies are needed in order to extend the storage time and to improve the hatching rate for the species.
- Subjects :
- Ethylene Glycol
Sucrose
Embryo, Nonmammalian
Cryobiology
Cryoprotectant
Embryonic Development
Aquaculture
Cryopreservation
chemistry.chemical_compound
Cryoprotective Agents
Animal science
Animals
Catfishes
biology
Hatching
Methanol
Rhinelepis aspera
Cell Biology
biology.organism_classification
chemistry
Biochemistry
Ethylene glycol
Brazil
Developmental Biology
Subjects
Details
- ISSN :
- 14698730 and 09671994
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Zygote
- Accession number :
- edsair.doi.dedup.....aa48e803fb598f5de4f9a88454216b00