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Expression of the E3 SUMO-1 ligases PIASx and PIAS1 during spermatogenesis in the rat
- Source :
- Gene Expression Patterns. 3:301-308
- Publication Year :
- 2003
- Publisher :
- Elsevier BV, 2003.
-
Abstract
- PIASx and PIAS1, two members of the conserved protein inhibitor of activated STAT (PIAS) family, are able to interact with and modulate activities of several distinct nuclear proteins, including androgen receptor (AR). PIASx and PIAS1 also function as E3-type ligases in small ubiquitin-related modifier 1 modifications. To gain better insight into the physiological roles of PIASx and PIAS1 in vivo, their cell-type specific expression and regulation were analyzed during testicular development and spermatogenesis in the rat. The expression of both PIASx and PIAS1 was low or undetectable in newborn rats. PIASx mRNA started to accumulate after day 20 of postnatal life, whereas expression of PIAS1 mRNA increased around day 30 after birth. In the adult rat testis, both PIASx and PIAS1 mRNA were present in Sertoli cells and in germ cells in the seminiferous epithelium at all stages. However, PIASx mRNA was more abundant in spermatocytes than in other cell types, whereas higher levels of PIAS1 mRNA were detected in late spermatocytes and round spermatids than in early spermatocytes. Since PIASx and PIAS1 accumulate in developing male germ cells, their regulatory functions are not only restricted to AR in Sertoli cells, but they also participate in molecular processes during meiosis.
- Subjects :
- Male
Cell type
Ubiquitin-Protein Ligases
Spermatocyte
Ligases
03 medical and health sciences
Testis
Genetics
medicine
Animals
Protein inhibitor of activated STAT
Spermatogenesis
Molecular Biology
In Situ Hybridization
030304 developmental biology
Cell Nucleus
0303 health sciences
Messenger RNA
biology
Gene Expression Profiling
030302 biochemistry & molecular biology
Proteins
Sertoli cell
Immunohistochemistry
Protein Inhibitors of Activated STAT
Molecular biology
Rats
Cell biology
Ubiquitin ligase
Androgen receptor
medicine.anatomical_structure
Protein Biosynthesis
biology.protein
Developmental Biology
Subjects
Details
- ISSN :
- 1567133X
- Volume :
- 3
- Database :
- OpenAIRE
- Journal :
- Gene Expression Patterns
- Accession number :
- edsair.doi.dedup.....aa42d4c03fcfff38fd825971cb48ee1b