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Evaluation of a new solid media specimen transport card for high risk HPV detection and cervical cancer prevention
- Source :
- Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology. 76
- Publication Year :
- 2015
-
Abstract
- Background Solid media transport can be used to design adaptable cervical cancer screening programs but currently is limited by one card with published data. Objective To develop and evaluate a solid media transport card for use in high-risk human papillomavirus detection (HR–HPV). Study design The Preventative Oncology International (POI) card was constructed using PK 226 paper ® treated with cell-lysing solution and indicating dye. Vaginal samples were applied to the POI card and the indicating FTA (iFTA) elute card. A cervical sample was placed in liquid media. All specimens were tested for HR–HPV. Color change was assessed at sample application and at card processing. Stability of the POI card and iFTA elute card was tested at humidity. Results 319 women were enrolled. Twelve women had at least one insufficient sample with no difference between media ( p = 0.36). Compared to liquid samples, there was good agreement for HR-HPV detection with kappa of 0.81 (95% CI 0.74–0.88) and 0.71 (95% CI 0.62–0.79) for the POI and iFTA elute card respectively. Sensitivity for ≥CIN2 was 100% (CI 100–100%), 95.1% (CI 92.7–97.6%), and 93.5% (CI 90.7–96.3%) for the HR–HPV test from the liquid media, POI card, and iFTA elute card respectively. There was no color change of the POI card noted in humidity but the iFTA elute card changed color at 90% humidity. Conclusions The POI card is suitable for DNA transport and HR–HPV testing. This card has the potential to make cervical cancer screening programs more affordable worldwide.
- Subjects :
- 0301 basic medicine
Adult
medicine.medical_specialty
Insufficient Sample
Uterine Cervical Neoplasms
Cervical cancer screening
Sensitivity and Specificity
Specimen Handling
03 medical and health sciences
0302 clinical medicine
Virology
Internal medicine
Medicine
Humans
Human papillomavirus
Early Detection of Cancer
Vaginal Smears
business.industry
Papillomavirus Infections
Middle Aged
Uterine Cervical Dysplasia
Solid medium
030104 developmental biology
Infectious Diseases
High risk hpv
030220 oncology & carcinogenesis
Cervical cancer prevention
DNA, Viral
Vagina
Female
business
Subjects
Details
- ISSN :
- 18735967
- Volume :
- 76
- Database :
- OpenAIRE
- Journal :
- Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
- Accession number :
- edsair.doi.dedup.....aa2a3cefa0712e00fae77119f7f9a84f