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Mouse Lymphoma Thymidine Kinase Gene Mutation Assay: International Workshop on Genotoxicity Tests Workgroup Report—Plymouth, UK 2002

Authors :
Michael R. O’Donovan
George Bolcsfoldi
Martha M. Moore
Robert Preston
Stephan Kirchner
Michael D. Fellows
Paul Kirby
Richard H.C. San
Ajit K. Thakur
Brian Myhr
Takashi Omori
Isao Yoshimura
Wolfgang Muster
Masamitsu Honma
Maria A. Cifone
B. Bhaskar Gollapudi
Peter Jenkinson
Robert R. Delongchamp
Shinobu Wakuri
Julie Clements
Marie-Claude Ouldelhkim
Colin Riach
Jo Oliver
Kamala Pant
Leon F. Stankowski
Source :
Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 540:127-140
Publication Year :
2003
Publisher :
Elsevier BV, 2003.

Abstract

The Mouse Lymphoma Assay (MLA) Workgroup of the International Workshop on Genotoxicity Tests (IWGT) met on June 28th and 29th, 2002, in Plymouth, England. This meeting of the MLA group was devoted to discussing the criteria for assay acceptance and appropriate approaches to data evaluation. Prior to the meeting, the group conducted an extensive analysis of data from both the microwell and soft agar versions of the assay. For the establishment of criteria for assay acceptance, 10 laboratories (6 using the microwell method and 4 using soft agar) provided data on their background mutant frequencies, plating efficiencies of the negative/vehicle control, cell suspension growth, and positive control mutant frequencies. Using the distribution curves generated from this data, the Workgroup reached consensus on the range of values that should be used to determine whether an individual experiment is acceptable. In order to establish appropriate approaches for data evaluation, the group used a number of statistical methods to evaluate approximately 400 experimental data sets from 10 laboratories entered into a database created for the earlier MLA Workshop held in New Orleans [Environ. Mol. Mutagen. 40 (2002) 292]. While the Workgroup could not, during this meeting, make a final recommendation for the evaluation of data, a general strategy was developed and the Workgroup members agreed to evaluate this new proposed approach using their own laboratory data. This evaluation should lead to a consensus global approach for data evaluation in the near future.

Details

ISSN :
13835718
Volume :
540
Database :
OpenAIRE
Journal :
Mutation Research/Genetic Toxicology and Environmental Mutagenesis
Accession number :
edsair.doi.dedup.....a9fa36bfb90fe6743c29df407360e734
Full Text :
https://doi.org/10.1016/j.mrgentox.2003.07.003