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Upregulation of recepteur d'origine nantais tyrosine kinase and cell invasiveness via early growth response-1 in gastric cancer cells
- Source :
- Journal of Cellular Biochemistry. 113:1217-1223
- Publication Year :
- 2012
- Publisher :
- Wiley, 2012.
-
Abstract
- Abnormal accumulation and activation of the recepteur d'origine nantais (RON) has been implicated in carcinogenesis of epithelial tumors. RON expression was induced by the tumor promoter, phorbol 12-myristate 13-acetate (PMA), in gastric adenocarcinoma AGS cells. Studies with deleted and site-directed mutagenesis of Egr-1 promoter and with expression vectors encoding Egr-1 confirmed that Egr-1 is essential for RON expression. In addition, AGS cells pretreated with PMA showed remarkably enhanced invasiveness, which was partially abrogated by siRNA-targeted RON and Egr-1. These results suggest that tumor promoter induces RON expression via Egr-1, which, in turn, stimulates cell invasiveness in AGS cells. J. Cell. Biochem. 113: 1217–1223, 2012. © 2011 Wiley Periodicals, Inc.
- Subjects :
- Chromatin Immunoprecipitation
medicine.medical_specialty
Blotting, Western
Cell
Adenocarcinoma
Biology
medicine.disease_cause
Biochemistry
chemistry.chemical_compound
Downregulation and upregulation
Stomach Neoplasms
Cell Line, Tumor
Internal medicine
medicine
Humans
Neoplasm Invasiveness
RNA, Small Interfering
Promoter Regions, Genetic
Molecular Biology
DNA Primers
Early Growth Response Protein 1
Expression vector
Base Sequence
Reverse Transcriptase Polymerase Chain Reaction
Mutagenesis
Receptor Protein-Tyrosine Kinases
Cell Biology
Up-Regulation
body regions
Endocrinology
medicine.anatomical_structure
chemistry
Cancer cell
Mutagenesis, Site-Directed
Cancer research
Phorbol
Carcinogenesis
Tyrosine kinase
hormones, hormone substitutes, and hormone antagonists
Subjects
Details
- ISSN :
- 07302312
- Volume :
- 113
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular Biochemistry
- Accession number :
- edsair.doi.dedup.....a9f80269556690924a95d3405f20ca87