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36-kDa Annexin A3 Isoform Negatively Modulates Lipid Storage in Clear Cell Renal Cell Carcinoma Cells
- Source :
- The American journal of pathology, 190 (2020): 2317–2326. doi:10.1016/j.ajpath.2020.08.008, info:cnr-pdr/source/autori:Bombelli, Silvia; Torsello, Barbara; De Marco, Sofia; Lucarelli, Giuseppe; Cifola, Ingrid; Grasselli, Chiara; Strada, Guido; Bovo, Giorgio; Perego, Roberto A.; Bianchi, Cristina/titolo:36-kDa Annexin A3 Isoform Negatively Modulates Lipid Storage in Clear Cell Renal Cell Carcinoma Cells/doi:10.1016%2Fj.ajpath.2020.08.008/rivista:The American journal of pathology (Print)/anno:2020/pagina_da:2317/pagina_a:2326/intervallo_pagine:2317–2326/volume:190
- Publication Year :
- 2020
- Publisher :
- Elsevier Inc., 2020.
-
Abstract
- The adipocyte-like morphology of clear cell renal cell carcinoma (ccRCC) cells results from a grade-dependent neutral lipid accumulation; however, the molecular mechanism and role in renal cancer progression have yet to be clarified. ccRCC shows a gene expression signature consistent with adipogenesis, and the phospholipid-binding protein annexin A3 (AnxA3), a negative regulator of adipocyte differentiation, is down-regulated in RCC and shows a differential expression pattern for two isoforms of 36 and 33 kDa. Using primary cell cultures and cell lines, we investigated the involvement of AnxA3 isoforms in lipid storage modulation of ccRCC cells. We found that the increased accumulation of lipids into ccRCC cells correlated with a decrease of the 36/33 isoform ratio. Treatment with adipogenic medium induced a significant increment of lipid storage in ccRCC cells that had a low 36-kDa AnxA3 expression and 36/33 ratio. The 36-kDa AnxA3 silencing in ccRCC cells increased lipid storage induced by adipogenic medium. These data suggest that 36-kDa AnxA3 negatively modulates the response to adipogenic treatment and may act as negative regulator of lipid storage in ccRCC cells. The subcellular distribution of AnxA3 in the cellular endocytic compartment suggests its involvement in modulation of vesicular trafficking, and it might serve as a putative mechanism of lipid storage regulation in ccRCC cells, opening novel translational outcomes.
- Subjects :
- Male
lipid storage
0301 basic medicine
Gene isoform
renal cell carcinoma, annexin A3, lipid storage
Pathology and Forensic Medicine
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Cell Line, Tumor
Adipocyte
medicine
Humans
Gene silencing
Carcinoma, Renal Cell
Annexin A3
Aged
ccRCC
isoforms
Lipid metabolism
Lipid Metabolism
medicine.disease
Kidney Neoplasms
Neoplasm Proteins
Cell biology
Isoenzymes
Clear cell renal cell carcinoma
030104 developmental biology
chemistry
Cell culture
Adipogenesis
030220 oncology & carcinogenesis
Female
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- The American journal of pathology, 190 (2020): 2317–2326. doi:10.1016/j.ajpath.2020.08.008, info:cnr-pdr/source/autori:Bombelli, Silvia; Torsello, Barbara; De Marco, Sofia; Lucarelli, Giuseppe; Cifola, Ingrid; Grasselli, Chiara; Strada, Guido; Bovo, Giorgio; Perego, Roberto A.; Bianchi, Cristina/titolo:36-kDa Annexin A3 Isoform Negatively Modulates Lipid Storage in Clear Cell Renal Cell Carcinoma Cells/doi:10.1016%2Fj.ajpath.2020.08.008/rivista:The American journal of pathology (Print)/anno:2020/pagina_da:2317/pagina_a:2326/intervallo_pagine:2317–2326/volume:190
- Accession number :
- edsair.doi.dedup.....a9ca6e363238f8cd174a004206d77bde