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The involvement of protein kinase C in the regulation of choline cotransport in Limulus
- Source :
- Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 123:255-261
- Publication Year :
- 1999
- Publisher :
- Elsevier BV, 1999.
-
Abstract
- The involvement of protein kinase C (PKC) in the regulation of [3H]choline cotransport was studied in Limulus brain hemi-slice preparations. The PKC activators, phorbol 12-myristate 13-acetate (PMA) or phorbol 12,13-dibutyrate (PDBu), significantly decreased [3H]choline cotransport. Conversely, the PKC inhibitors, staurosporine (STAURO) and polymyxin B (PMB), each increased [3H]choline cotransport. These PKC inhibitors prevented the phorbol ester-induced reduction of transport. Both the PMA induced decrease and the STAURO induced increase in [3H]choline cotransport were paralleled by respective and comparable changes in [3H]hemicholinium-3 (HC-3) specific binding. Pre-exposure of brain hemi-slices to elevated potassium chloride (120 mM KCl) resulted in a doubling of [3H]choline cotransport and [3H]HC-3 binding. The enhancement of [3H]choline cotransport by STAURO and antecedent 120 mM KCl treatment were additive. PMA did not significantly alter elevated potassium stimulated transport. Moreover, arachidonyltrifluoromethyl ketone (AACOCF3) and quinacrine (QUIN), both phospholipase A2 (PLA2) inhibitors, markedly decreased enhanced [3H]choline transport and [3H]HC-3 binding induced by antecedent exposure to depolarizing concentrations of potassium. These results suggest that PKC and PLA2 are involved in the regulation of [3H]choline cotransport but at different regulatory sites.
- Subjects :
- medicine.medical_specialty
Physiology
Cholinergic Agents
Arachidonic Acids
Tritium
Nervous System
Biochemistry
Phospholipases A
Choline
Potassium Chloride
chemistry.chemical_compound
Organ Culture Techniques
Phospholipase A2
Internal medicine
Horseshoe Crabs
medicine
Animals
Staurosporine
Enzyme Inhibitors
Molecular Biology
Phorbol 12,13-Dibutyrate
Protein Kinase C
Protein kinase C
Phospholipase A
biology
Brain
Biological Transport
Hemicholinium 3
Phospholipases A2
Endocrinology
chemistry
Quinacrine
Carcinogens
Phorbol
biology.protein
Tetradecanoylphorbol Acetate
Choline transport
Carrier Proteins
Cotransporter
medicine.drug
Subjects
Details
- ISSN :
- 10956433
- Volume :
- 123
- Database :
- OpenAIRE
- Journal :
- Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
- Accession number :
- edsair.doi.dedup.....a9c04290ce0324f2f7bfd3d34cbd0369