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Preformulation Study of Highly Purified Inactivated Polio Vaccine, Serotype 3
- Source :
- Journal of Pharmaceutical Sciences. 103:140-151
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- To improve the effectiveness of the polio vaccination campaign, improvements in the Thermal Stability of IPV3 at pH 7 as the vaccine are being investigated. Here, inactivated polio vaccine, serotype 3 (IPV3) was characterized via a number of biophysical techniques. The size was characterized by transmission electronic microscopy and light scattering. The capsid protein conformation was evaluated by intrinsic fluorescence and circular dichroism (CD), and the D-antigen content by enzyme-linked immunosorbent assay (ELISA). The pH thermal stability of IPV3 (pH 3.0ā8.0; 10°Cā87.5°C) was evaluated by fluorescence, CD, and static light scattering. The transition temperatures reflect the responses, respectively, of tertiary structure, secondary structure, and size to applied thermal stress. The data were summarized as empirical phase diagrams, and the most stable conditions were found to be pH 7.0 with temperature lower than 40°C. CD detected a higher transition temperature for capsid protein than that for RNA. The effects of certain excipients on IPV3 thermal stability and antigen content were evaluated. The results of their effects, based on intrinsic fluorescence and ELISA, were in good agreement, suggesting the feasibility of applying intrinsic fluorescence as a high-throughput tool for formulation development. The study improves the understanding of IPV3 thermal stability, and provides a starting point for future formulation development of IPV3 and other serotypes. © 2013 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 103:140ā151, 2014
- Subjects :
- Circular dichroism
Chromatography
Chemistry
Chemistry, Pharmaceutical
Pharmaceutical Science
Viral Vaccines
Hydrogen-Ion Concentration
Fluorescence
Protein Structure, Secondary
Polio Vaccination
Protein tertiary structure
Protein Structure, Tertiary
Excipients
Poliovirus
Crystallography
Capsid
Transition Temperature
Capsid Proteins
Static light scattering
Thermal stability
Antigens, Viral
Protein secondary structure
Poliomyelitis
Subjects
Details
- ISSN :
- 00223549
- Volume :
- 103
- Database :
- OpenAIRE
- Journal :
- Journal of Pharmaceutical Sciences
- Accession number :
- edsair.doi.dedup.....a990d19db429a57841b226320a5b07de