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Feasibility of next-generation sequencing (Oncomine™ DX Target Test) for the screening of oncogenic mutations in advanced non-small-cell lung cancer patients
- Source :
- Japanese journal of clinical oncology. 51(7)
- Publication Year :
- 2020
-
Abstract
- Background The Oncomine™ Dx Target Test based on next-generation sequencing has been approved for the screening of oncogenic mutations in advanced non-small-cell lung cancer patients. Methods We assessed the tissue sample factors that affect the success rate of Oncomine™ Dx Target Test companion diagnostics and the feasibility of using biopsy specimens for Oncomine™ Dx Target Test companion diagnostics in advanced non-small-cell lung cancer patients. Results Ninety-nine biopsy samples were subjected to genetic testing using the Oncomine™ Dx Target Test companion diagnostics to detect v-raf murine sarcoma viral oncogene homologue B1 mutations (Cohort 1), and 136 biopsy samples were examined using Oncomine™ Dx Target Test companion diagnostics for the detection of multiple oncogenic mutations (Cohort 2) between July 2018 and April 2020. We retrospectively collected clinical and pathological data, including tissue size and tumour cell content. The success rate was 77% (76/99) in Cohort 1 and 93% (127/136) in Cohort 2. In Cohort 1, the success rate was significantly associated with the tumour cell content: the success rate was 63% for samples with a tumour cell content of Conclusions Tissue size and tumour cell content were significantly associated with the success rate of Oncomine™ Dx Target Test companion diagnostics.
- Subjects :
- Adult
Aged, 80 and over
Male
Cancer Research
Lung Neoplasms
High-Throughput Nucleotide Sequencing
General Medicine
Middle Aged
03 medical and health sciences
0302 clinical medicine
030228 respiratory system
Oncology
030220 oncology & carcinogenesis
Carcinoma, Non-Small-Cell Lung
Mutation
Animals
Feasibility Studies
Humans
Mass Screening
Radiology, Nuclear Medicine and imaging
Female
Genetic Testing
Early Detection of Cancer
Aged
Retrospective Studies
Subjects
Details
- ISSN :
- 14653621
- Volume :
- 51
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Japanese journal of clinical oncology
- Accession number :
- edsair.doi.dedup.....a98cd9cf63fca7c94b23befca44367da