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Heterologous Priming-Boosting Immunization of Cattle withMycobacterium tuberculosis85A Induces Antigen-Specific T-Cell Responses
- Source :
- Infection and Immunity. 71:6906-6914
- Publication Year :
- 2003
- Publisher :
- American Society for Microbiology, 2003.
-
Abstract
- Heterologous priming-boosting vaccination regimens involving priming with plasmid DNA antigen constructs and inoculating (boosting) with the same recombinant antigen expressed in replication-attenuated poxviruses have recently been demonstrated to induce immunity, based on CD4+- and CD8+-T-cell responses, against several diseases in both rodents and primates. We show that similar priming-boosting vaccination strategies using the 85A antigen ofMycobacterium tuberculosisare effective in inducing antigen-specific gamma interferon-secreting CD4+and CD8+T cells, detected by a bovine enzyme-linked immunospot assay, inBos indicuscattle. T-cell responses induced by priming with either plasmid DNA or fowlpox virus 85A constructs were enhanced by boosting with modified vaccinia virus Ankara expressing the same antigen administered intradermally. On the basis of the data, it appears that intradermal priming was more effective than intramuscular delivery of the priming dose for boosting with the modified vaccinia virus Ankara strain in cattle. Using either fowlpox virus or DNA priming, there was a significant bias toward induction of CD4+- rather than CD8+-T-cell responses. These data illustrate the general applicability of priming-boosting vaccination strategies for induction of antigen-specific T-cell responses and suggest that the method may be useful for development of veterinary vaccines.
- Subjects :
- CD4-Positive T-Lymphocytes
Cellular immunity
viruses
Immunology
Immunization, Secondary
Cattle Diseases
Priming (immunology)
Heterologous
CD8-Positive T-Lymphocytes
Biology
Lymphocyte Activation
complex mixtures
Microbiology
Virus
Interferon-gamma
chemistry.chemical_compound
Antigen
Vaccines, DNA
Animals
Tuberculosis Vaccines
Antigens, Bacterial
Fowlpox virus
Mycobacterium tuberculosis
Virology
Recombinant Proteins
Infectious Diseases
chemistry
Microbial Immunity and Vaccines
Cattle
Immunization
Parasitology
Vaccinia
Peptides
Tuberculosis vaccines
Tuberculosis, Bovine
Acyltransferases
Plasmids
Subjects
Details
- ISSN :
- 10985522 and 00199567
- Volume :
- 71
- Database :
- OpenAIRE
- Journal :
- Infection and Immunity
- Accession number :
- edsair.doi.dedup.....a982616d96daaf27a31256cbb8be72bb