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Signalling and bioactive metabolites from Streptomyces sp. RK44
- Source :
- Digital.CSIC. Repositorio Institucional del CSIC, instname, Molecules, Volume 25, Issue 3, Molecules, Vol 25, Iss 3, p 460 (2020)
- Publication Year :
- 2020
- Publisher :
- Molecular Diversity Preservation International, 2020.
-
Abstract
- Streptomyces remains one of the prolific sources of structural diversity, and a reservoir to mine for novel natural products. Continued screening for new Streptomyces strains in our laboratory led to the isolation of Streptomyces sp. RK44 from the underexplored areas of Kintampo waterfalls, Ghana, Africa. Preliminary screening of the metabolites from this strain resulted in the characterization of a new 2-alkyl-4-hydroxymethylfuran carboxamide (AHFA) 1 together with five known compounds, cyclo-(L-Pro-Gly) 2, cyclo-(L-Pro-L-Phe) 3, cyclo-(L-Pro-L-Val) 4, cyclo-(L-Leu-Hyp) 5, and deferoxamine E 6. AHFA 1, a methylenomycin (MMF) homolog, exhibited anti-proliferative activity (EC50 = 89.6 &micro<br />M) against melanoma A2058 cell lines. This activity, albeit weak is the first report amongst MMFs. Furthermore, the putative biosynthetic gene cluster (ahfa) was identified for the biosynthesis of AHFA 1. DFO-E 6 displayed potent anti-plasmodial activity (IC50 = 1.08&micro<br />M) against P. falciparum 3D7. High-resolution electrospray ionization mass spectrometry (HR ESIMS) and molecular network assisted the targeted-isolation process, and tentatively identified six AHFA analogues, 7&ndash<br />12 and six siderophores 13&ndash<br />18.
- Subjects :
- Siderophore
signalling molecules
Streptomyces sp. RK44
medicine.drug_class
Electrospray ionization
education
Pharmaceutical Science
Carboxamide
methylenomycin
01 natural sciences
Streptomyces
Analytical Chemistry
lcsh:QD241-441
03 medical and health sciences
chemistry.chemical_compound
lcsh:Organic chemistry
Biosynthesis
Drug Discovery
Gene cluster
medicine
VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470
Antimalaria
Physical and Theoretical Chemistry
AHFA
030304 developmental biology
MMFs
0303 health sciences
biology
010405 organic chemistry
Chemistry
Organic Chemistry
biology.organism_classification
Methylenomycin
VDP::Mathematics and natural science: 400::Basic biosciences: 470
3. Good health
0104 chemical sciences
Anticancer
Biochemistry
Chemistry (miscellaneous)
Molecular Medicine
Bacteria
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Digital.CSIC. Repositorio Institucional del CSIC, instname, Molecules, Volume 25, Issue 3, Molecules, Vol 25, Iss 3, p 460 (2020)
- Accession number :
- edsair.doi.dedup.....a9807d99d75dbdb8c754dc1f75dfc611