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Low concentration of DMSO stabilizes the bilayer gel phase rather than the interdigitated gel phase in dihexadecylphosphatidylcholine membrane
- Source :
- Biochimica et Biophysica Acta (BBA) - Biomembranes. 1467:395-405
- Publication Year :
- 2000
- Publisher :
- Elsevier BV, 2000.
-
Abstract
- We have investigated effects of dimethylsulfoxide (DMSO) on the phase stability of multilamellar vesicles of the ether-linked 1,2-dihexadecyl-sn-glycero-3-phosphatidylcholine (DHPC-MLV), which is known to be in the interdigitated gel (LbetaI) phase in excess water at 20 degrees C. The results of X-ray diffraction experiments indicate that the DHPC membrane was in the Lbeta, phase at X> or =0.12 (X=mole fraction of DMSO in DMSO/water mixture). The result of differential scanning calorimetry indicate that the gel to liquid-crystalline phase transition temperature increased, but the LbetaI to Pbeta, phase transition temperature decreased with an increase in DMSO concentration. These results show that DMSO stabilizes the bilayer gel phase rather than the LbetaI phase at its low concentration. The solubility of phosphorylcholine, which is the same structure as the headgroup of DHPC, decreased with an increase in DMSO concentration, indicating that the interaction free energy of the hydrophilic segments of the membrane with solvents increases with an increase in DMSO concentration. On the basis of the thermodynamic analysis, the mechanism of the stabilization of the bilayer gel phase of DHPC-MLV by DMSO is discussed. The decrease in the repulsive interaction between the headgroups of the phospholipid induced by the low concentrations of DMSO in water plays an important role in this stabilization.
- Subjects :
- Dimethylsulfoxide
Phase transition
Lipid Bilayers
Biophysics
Analytical chemistry
Hydration
Calorimetry
In Vitro Techniques
Mole fraction
Biochemistry
Cryoprotective Agents
Differential scanning calorimetry
Drug Stability
X-Ray Diffraction
Phase (matter)
Dimethyl Sulfoxide
Solubility
Phospholipid membrane interface
Calorimetry, Differential Scanning
Chemistry
Bilayer
Phospholipid Ethers
Membranes, Artificial
Cell Biology
Interdigitated gel phase
Interaction free energy
Crystallography
Membrane
Liposomes
Thermodynamics
Gels
Subjects
Details
- ISSN :
- 00052736
- Volume :
- 1467
- Database :
- OpenAIRE
- Journal :
- Biochimica et Biophysica Acta (BBA) - Biomembranes
- Accession number :
- edsair.doi.dedup.....a96c14e39075f89d44b94d82d8d3523d