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Fusogenic activity of cationic lipids and lipid shape distribution
- Source :
- Cellular and Molecular Life Sciences. 67:483-494
- Publication Year :
- 2009
- Publisher :
- Springer Science and Business Media LLC, 2009.
-
Abstract
- Addition of co-lipids into cationic lipid formulations is considered as promoting cell delivery of DNA by enhancing fusion processes with cell membranes. Here, by combining FRET and confocal microscopy, we demonstrate that some cationic lipids do not require a co-lipid to fuse efficiently with cells. These cationic lipids are able to self-organize into bilayers that are stable enough to form liposomes, while presenting some destabilizing properties reminiscent of the conically shaped fusogenic co-lipid, DOPE. We therefore analyzed the resident lipid structures in cationic bilayers by molecular dynamics simulations, clustering the individual lipid structures into populations of similarly shaped molecules, as opposed to the classical approach of using the static packing parameter to define the lipid shapes. Comparison of fusogenic properties with these lipid populations suggests that the ratio of cylindrical versus conical lipid populations correlates with the ability to fuse with cell membranes.
- Subjects :
- Molecular Dynamics Simulation
Biology
law.invention
Fatty Acids, Monounsaturated
Mice
Cellular and Molecular Neuroscience
Molecular dynamics
Confocal microscopy
law
Cations
Cell Line, Tumor
Fluorescence Resonance Energy Transfer
Animals
Molecule
Lipid bilayer phase behavior
Molecular Biology
Pharmacology
Liposome
Microscopy, Confocal
Phosphatidylethanolamines
Cell Membrane
Gene Transfer Techniques
Cationic polymerization
Cell Biology
Lipids
Cell biology
Quaternary Ammonium Compounds
Membrane
Förster resonance energy transfer
Liposomes
Molecular Medicine
lipids (amino acids, peptides, and proteins)
Subjects
Details
- ISSN :
- 14209071 and 1420682X
- Volume :
- 67
- Database :
- OpenAIRE
- Journal :
- Cellular and Molecular Life Sciences
- Accession number :
- edsair.doi.dedup.....a969d7d377bddeee3af997931feaaeda
- Full Text :
- https://doi.org/10.1007/s00018-009-0197-x