Zika detection: comparison of methodologies

Made available in DSpace on 2018-11-26T15:47:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-01-01. Added 1 bitstream(s) on 2019-10-09T18:25:22Z : No. of bitstreams: 1 S1517-83822018000100144.pdf: 591481 bytes, checksum: 392b744e5bdcc30f75d329a1ae39d213 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Many countries in the Americas have detected local transmission of multiple arboviruses that cause febrile illnesses. Therefore, laboratory testing has become an important tool for confirming the etiology of these diseases. The present study aimed to compare the sensitivity and specificity of three different Zika virus detection assays. One hundred serum samples from patients presenting with acute febrile symptoms were tested using a previously reported TaqMan (R) RT-qPCR assay. We used a SYBR (R) Green RT-qPCR and a conventional PCR methodologies to compare the results. Of the samples that were determined to be negative by the TaqMan (R) RT-qPCR assay, 100% (Kappa = 0.670) were also found to be negative by SYBR (R) Green RT-qPCR based on Tm comparison; however, 14% (Kappa = 0.035) were found to be positive by conventional PCR followed by agarose gel electrophoresis. The differences between the ZIKV strains circulating worldwide and the low viremia period can compromise diagnostic accuracy and thereby the accuracy of outbreak data. Therefore, improved assays are required to improve the diagnosis and surveillance of arbovirus. (C) 2017 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. Fac Med Sao Jose do Rio Preto FAMERP, Sao Jose Do Rio Preto, SP, Brazil Univ Estadual Paulista, Inst Biociencias Botucatu, Lab Virol, Botucatu, SP, Brazil Univ Nova Lisboa, GHTM, Lisbon, Portugal Prefeitura Sao Jose do Rio Preto, Dept Vigilancia Saude, Sao Jose Do Rio Preto, SP, Brazil Univ Estadual Paulista, Inst Biociencias Botucatu, Lab Virol, Botucatu, SP, Brazil FAPESP: 2013/21719-3