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Genome-wide analysis of water-stress-responsive microRNA expression profile in tobacco roots
- Source :
- Functionalintegrative genomics. 14(2)
- Publication Year :
- 2013
-
Abstract
- MicroRNAs (miRNAs) play a pivotal role in post-transcriptional regulation of gene expression in plants. In this study, we investigate miRNAs in an agronomically important common tobacco in China, named Honghua Dajinyuan (a drought-tolerant cultivar). Here, we report a comprehensive analysis of miRNA expression profiles in mock-treat grown (CK) and 20 % polyethylene glycol-grown (PEG-grown) tobacco roots using a high-throughput sequencing approach. A total of 656 unique miRNAs representing 53 miRNA families were identified in the two libraries, of which 286 unique miRNAs representing 162 microRNAs were differentially expressed. In addition, nine differentially expressed microRNAs selected from different expressed miRNA family with high abundance were subjected to further analysis and validated by quantitative real-time PCR (Q-PCR). In addition, the expression pattern of these identified candidate conserved miRNA and target genes of three identified miRNA (nta-miR172b, nta-miR156i, and nta-miR160a) were also validated by Q-PCR. Gene ontology (GO) enrichment analysis suggests that the putative target genes of these differentially expressed miRNAs are involved in metabolic process and response to stimulus. In particular, 25 target genes are involved in regulating plant hormone signal transduction and metabolism, indicating that these association microRNAs may play important regulatory roles in responding to PEG resistance. Moreover, this study adds a significant number of novel miRNAs to the tobacco miRNome.
- Subjects :
- Biology
Genome
Plant Roots
Polyethylene Glycols
Plant Growth Regulators
Gene Expression Regulation, Plant
Stress, Physiological
microRNA
Tobacco
Genetics
Genomic library
Gene
Gene Library
Plant Proteins
Regulation of gene expression
Dehydration
Gene Expression Profiling
RNA
Gene Expression Regulation, Developmental
General Medicine
MicroRNA Expression Profile
Gene expression profiling
MicroRNAs
RNA, Plant
Genome, Plant
Signal Transduction
Subjects
Details
- ISSN :
- 14387948
- Volume :
- 14
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Functionalintegrative genomics
- Accession number :
- edsair.doi.dedup.....a92f781e949e8c0dcfcb964fea181821