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Efficient Epstein-Barr Virus Progeny Production Mediated by Cancer-Derived LMP1 and Virally-Encoded microRNAs
- Source :
- Microorganisms, Vol 7, Iss 5, p 119 (2019), Microorganisms, Volume 7, Issue 5
- Publication Year :
- 2019
- Publisher :
- MDPI AG, 2019.
-
Abstract
- Epstein-Barr virus (EBV) genomes, particularly their latent genes, are heterogeneous among strains. The heterogeneity of EBV-encoded latent membrane protein 1 (LMP1) raises the question of whether there are functional differences between LMP1 expressed by cancer-associated EBV and that by non-cancerous strains. Here, we used bacterial artificial chromosome (BAC)-cloned EBV genomes retaining all virally encoded microRNA (miRNA) genes to investigate the functions of cancer-derived LMP1 in the context of the EBV genome. HEK293 cells were stably transfected with EBV-BAC clone DNAs encoding either nasopharyngeal carcinoma (NPC)-derived CAO-LMP1 (LMP1CAO) or LMP1 from a prototype B95-8 strain of EBV (LMP1B95-8). When an EBV-BAC clone DNA encoding LMP1CAO was stably transfected into HEK293 cells, it generated many more stable transformants than the control clone encoding LMP1B95-8. Furthermore, stably transfected HEK293 cells exhibited highly efficient production of progeny virus. Importantly, deletion of the clustered viral miRNA genes compromised the ability to produce progeny viruses. These results indicate that cancer-derived LMP1 and viral miRNAs together are necessary for efficient production of progeny virus, and that the resulting increase in efficiency contributes to EBV-mediated epithelial carcinogenesis.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
viruses
Clone (cell biology)
Biology
medicine.disease_cause
Microbiology
Genome
Virus
Article
03 medical and health sciences
0302 clinical medicine
Virology
hemic and lymphatic diseases
medicine
otorhinolaryngologic diseases
Nasopharyngeal carcinoma
Epstein-Barr virus
Gene
lcsh:QH301-705.5
LMP1
Bacterial artificial chromosome
microRNA
Transfection
Epstein–Barr virus
stomatognathic diseases
030104 developmental biology
lcsh:Biology (General)
030220 oncology & carcinogenesis
BART
Carcinogenesis
Subjects
Details
- Language :
- English
- ISSN :
- 20762607
- Volume :
- 7
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Microorganisms
- Accession number :
- edsair.doi.dedup.....a9294b45e79b232babbb97aebce2a729